Objective To investigate the validation and safety of domestic non-absorbable disintegrating anastomat for enteroanastomosis in vivo. Methods Compared with Valtrac biofragmentale anastomosis rings ( BARs), cytotoxicity of domestic non-absorbable disintegrating anastomat in vitro was tested by MTT. Operative complications, time for anastomat discharging and anastomotic healing were also compared through zoopery. Results Optical density of domestic non-absorbable disintegrating anastomat and Valtrac BARs by MTT were 0.616±0.028 and 0.601 ± 0.048, respectively ( P ＞ 0. 05 ). No death, anastomotic leaks, anastomotic stricture, bleeding or ileus occurred in both groups. Domestic non-absorbable disintegrating anastomat fragmented at a mean time of (14.1 ± 1.4) days (ranged from 12 to 17 days) postoperatively, while BARs fragmented at a mean time of ( 14. 5 ± 1.7 ) days ( ranged from 12 to 16 days) (P＞0.05). Favourable anastomotic healing was detected by histological examination. Conclusion Domestic non-absorbable disintegrating anastomat for enteroanastomosis had been proved the same validation and safety as Valtrac BARs in zoopery, without any significant cytotoxicity in vitro.