Objective To investigate the effects of bile on chloride channel protein-2 (CLC-2)and permeability of intestinal epithelium cell.Methods Rat intestinal epithelium cell line IEC-6 was cultured.They were exposed to 5.0％,1.0％,0.1％ rat bile and chloride channel agonist Lubiprostone.After 20 h cultured,transepithelial electrical resistance (TER) of the monostratal cells was measured.The change of CLC-2 and zonula occludens-1 (ZO-1) were examined by Western blotting,and the images were analysed quantitatively.Results The TEER of 5％ group was most lower.The average relative gradations of Western blotting images in 5.0％ group and 1.0％ were lower obviously than those in control group (0.30 ± 0.05,0.37 ± 0.08 vs.0.56 ± 0.08) ( all P ＜ 0.05 ).The relative gradations of ZO-1 were decreaseded only in 5.0％ group.After Lubiprostone added,the TER in 5.0％ group was upgraded (451.3 ± 60.5 )Ω·cm2,and the average relative gradations of ZO-1 (0.32 ± 0.04).Conclusion High concentration of bile destroied enterocyte chloride channel and tight junction protein,increase epithelium permeabihty.