Expression and significance of proto-oncogene PIM-1 in bladder transitional cell carcinoma
CHEN Jun-xing, MAO Xiao-peng, HUANG Bin, RAO Zhi-jun, ZHUANG Jin-tao, QIU Shao-peng
Published 2011-12-08
Cite as Chin J Exp Surg, 2011,28(12): 2092-2094. DOI: 10.3760/cma.j.issn.1001-9030.2011.12.021
Abstract
Objective To study the expression and significance of PIM-1 in bladder transitional cell carcinoma.Methods Expression and localization of PIM-1 in human normal and malignant bladder specimens were examined by Immunohistochemistry and the PIM-1 staining score was also compared with several clinicopathologic parameters; then PIM-1 expression was also validated in five bladder cancer cell lines by western blotting and immunohistochemistry assays.Subsequent knockdown of PIM-1 was achieved by lentivirus encoding small interfering RNA,and the effect of PIM-1 on bladder cell survival was further assessed by colony formation assays,finally the anti-apoptosis protein bcl-2 and p-BAD were examined by WB.Results When compared with normal epithelium,PIM-1 was overexpressed in bladder cancer epithelium (2/21 to 38/45) (Pvalue < 0.01 ),and the expression level was even higher in invasive bladder cancer than Non-invasive bladder cancer specimens ( 19/20 to 19/25 ) (P value <0.01 ).PIM-1 was also detected in all the bladder cancer cell lines examined.Moreover,the knockdown of PIM-1 significantly inhibited bladder cancer cell growth which associated with the reduction of bcl-2 and p-BAD.Conclusion PIM-1 is up-regulated in bladder transitional cell carcinoma specimens and associated with the tumor stage,more importantly PIM-1 is much higher in invasive bladder transitional cell carcinoma than no-invasive bladder transitional cell carcinoma.Further more,PIM-1 is highly expressed in bladder cancer cell lines and downregulated PIM-1 can inhibit the growth of bladder cancer cells and is involve in the increase of apoptosis,therefore PIM-1 may play a role in bladder cancer initiation and progression which may be a potential candidate as target for novel therapy in bladder cancer.
Key words:
Bladder carcinoma; Kirase; PIM-1
Contributor Information
CHEN Jun-xing
Department of Urology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
MAO Xiao-peng
Department of Urology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
HUANG Bin
Department of Urology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
RAO Zhi-jun
Department of Urology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
ZHUANG Jin-tao
Department of Urology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
QIU Shao-peng
Department of Urology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China