A novel material to create pulmonary artery stenosis in a swine model
CHEN Ze-rui, WANG De, WU Xin, PAN Xiang-bin, HONG Liang, LI Shou-jun
Published 2012-08-08
Cite as Chin J Exp Surg, 2012,29(08): 1458-1459,封3. DOI: 10.3760/cma.j.issn.1001-9030.2012.08.012
Abstract
Objective To evaluate the effectiveness of autologous pericardium applied to pulmonary artery banding in a swine model.Methods In a swine model,pulmonary artery stenosis was created by autologous pericardium which made the left pulmonary artery banded to 30%-40% of its original diameter.Animals underwent right heart catheterization and angiography 4 weeks postoperatively,and balloon-expandable'stents were implanted through right ventricular outflow tract.The animals were sacrificed at 12th week following stenting and microscopic evaluation for histologic changes was made.Results Pulmonary artery banding was performed on 8 swines.The lumen diameter was decreased from (16.0 ± 0.4) mm to (6.4 ± 0.2 )mm ( P < 0.01 ),while the gradient inereased from median (range) 0 mm Hg to 35 ( 18-52 )mm Hg (1 mm Hg =0.133 kPa,P <0.01 ) following banding.One month later,the models were all survived.Compared to the instant result after banding,there was no significant difference in the lumen diameter ( P > 0.05) or the gradient (P > 0.05),and there was no banding dislodged and also no animal with severe left pulmonary artery hypoplasia or atresia.Conclusion Autologous pericardium as a banding to create an experimental pulmonary artery stenosis model appears safe and effective.
Key words:
Autologous pericardium; Pulmonary artery stenosis
Contributor Information
CHEN Ze-rui
Center for Pediatric Cardiac Surgery, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China
WANG De
Center for Pediatric Cardiac Surgery, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China
WU Xin
Center for Pediatric Cardiac Surgery, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China
PAN Xiang-bin
Center for Pediatric Cardiac Surgery, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China
HONG Liang
Center for Pediatric Cardiac Surgery, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China
LI Shou-jun
Center for Pediatric Cardiac Surgery, Fuwai Hospital, National Center for Cardiovascular Disease, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100037, China