Objective To evaluate the effects of small interfering RNA (siRNA) against protein kinase Cε (PKCε) gene on the proliferation,migration and invasion of human clear cell RCC cell line 769P cells.Methods PKCε-siRNA was transfected into the 769P cells.The mRNA and protein expression of PKCε was detected by using reverse transcription polymerase chain reaction (RT-PCR) and Western blotting,respectively.The proliferation of 769P cells was measured by methyl thiazol tetrazolium (MTT) assay and colony formation assay.Cell migration and invasion was assessed by using wound-healing assay and CHEMICON cell invasion assay,respectively.Results PKCε-siRNA efficiently down-regulated the PKCε expression,which resulted in inhibition of the proliferation,migration,and invasion of clear cell RCC cell line 769P.Conclusion PKCεis may be related with proliferation,migration and invasion of 769P cells,and may be a potential therapeutic target for clear cell RCC.