Objective To investigate the protection of non-heart-beating-donor lung with part liquid ventilation.Methods Thirty-six health SD rats were randomly divided into three groups,n =12:oxygen group (group C),salt water group (group B),and part liquid ventilation group (group A).Mechanical ventilation was done for 2 h after establishment of non-heart-beating donor lung model,and airway pressure was measured continuously during 2 h.After 2 h,P-V curve was measured.Resection the under leaves of the rats,observe the different pathological characteristics by electron microscopy of the lung.Right lung was taking irrigation,measurement of bronchial lung lavaged suffactant protein B,C (SP-B,the SP-C) concentrations.Cut the upper lobe toward the right lung and measurement SP-B and SP-C with real-time quantitative polymerase chain reaction (Real-time PCR) testing.Results The dynamic compliance and static compliance of group A are better than group B and C during 2 hours hot ischemia(P ＜0.05) ;Pathology organization score of group A is better than group B and C (P ＜ 0.05) ; SP-B and SP-C enzyme linked immunosorbent assay (ELISA) test and lung tissue of the Real-time PCR testing instructions protein and RNA content are difference,group A is higher than group B and C (P ＜ 0.05).Conclusion Part liquid ventilation protect pulmonary lung function and lung tissue well during heat during ischemia,and have very good protection effect to the SP-B and SP-C.