Preparation and cell compatibility of chitosan composite bioactive glass
Sun Chen, Zhu Shaobo, Jin Lin, Qi Baiwen, Zhang Tao, Maihemutijiang· Muhaimaiti
Published 2014-02-08
Cite as Chin J Exp Surg, 2014,31(02): 368-371. DOI: 10.3760/cma.j.issn.1001-9030.2014.02.052
Abstract
Objective To design a kind of bioactive glasses/chitosan (BG/CS) composite materials,and investigate their physico-chemical properties and cell compatibility.Methods 2.0% chitosan of hydrochloric acid solution and β-glycerophosphate on ice conditions in the 7∶1 ratio of well mixed.after CS/BG become 2.0∶ 1.0,1.0∶ 1.0,1.0∶1.5 gelatinous liquid stage.Save it in the-80 ℃ frige,formed by freeze-drying machine.After 20 kGy 60Go radiation sterilization get the sample.By scanning electron microscope,X-ray diffraction and Fourier infrared spectral analysis,DSC thermal analysis,its microstructure and composition,Calculate the stents' porosity,adopt ceramic test system of mechanical properties test.Inoculate the third-generation rabbit mesenchymal stem cells into the stents and use electron microscopy scanning the adherency of stents.Detect the proliferation of cells which are adhere to the stents.Evaluate the compatibility of the stents and cells.Results Synthetic CS/BG stents has the same size and geometric shapes with mould.The phenomenon of hydroxyapatite crystals gathered and carbon nanotube reunion was not detected.The porosity is 81%-89%,it is appropriate.The breaking strength is (4.90 ±0.63) mPa,Characteristic BG diffraction peak can be seen through X-ray diffraction figure technology.The absorption peaks of BG can be detected through fourier transform infrared spectroscopy.It indicate that BG is consist in the material.The third generation of rabbit rabbit mesenchymal stem cells adhere to the stent when cultivate for one day,some cells extends and put out locomotions.Training 7 days later,the cells grows well,and spread along the stent,it can be seen that cells polygonal and cell with material connected through slender locomotion.bone marrow mesenchymal stem cell grows well in the CS/BG stents.Conclusion The CS/BG stents can be preparate through the solution blending and freeze drying technology.The stents has good porosity,high mechanical strength and good compatibility with cells.It can be used for organization of bone tissue engineering.
Key words:
Bioactive glasses ; Chitosan; Tissue engineering; Bone
Contributor Information
Sun Chen
Department of Micro-orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
Zhu Shaobo
Department of Micro-orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
Jin Lin
Department of Micro-orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
Qi Baiwen
Department of Micro-orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
Zhang Tao
Department of Micro-orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430071, China
Maihemutijiang· Muhaimaiti
Department of Micro-orthopedics, Zhongnan Hospital of Wuhan University, Wuhan 430071, China