Objective To investigate the expression of long chain non-coding RNA (LncRNA) in hepatocellular carcinoma tissue and its clinical significance.Methods Real-time quantitative polymerase chain reaction (Real-time PCR) method was used to detect the expression of seven kinds of LncRNA associated with tumor [B cell integration cluster gene(BIC),deleted in lymphocytic leukemia 1 (DLEU1),small nucleolar RNA host genes 5 (SNHG5),insulin-like growth factor 2,antisense (IGF2AS),prostate cancer gene 3 (PCA3),Hypoxia inducible factor α (HIF-α) and Prostate cancer gene expression marker 1 (PCGEM1)] in liver cancer tissues and adjacent tissues (n =40).Results The expression of SNHG5,IGF2AS,PCA3,and HIF-α in liver cancer tissues and adjacent tissues was statistically significantly different (respectively P ＜ 0.01).IGF2AS was most significantly differentially expressed [HCC (10.910 ± 1.775) vs.paratumor (13.517 ± 3.077)],and its expression in liver cancer tissue was closely related to tumor number (P ＜ 0.05) and microvascular invasion (P ＜ 0.01).Cox multi-factor analysis showed that the expression of IGF2AS in tumor tissues was an independent prognostic factors of the liver disease-free survival.Conclusion The important role of IGF2AS played in HCC development and prognosis is worthy of further study.