Experimental study of vascularized to three dimensional printing of bionic human phalanx scaffold
Wang Wei, Alimujiang·Abulaiti, Aierken·Rehemutula, Aihemaitijiang·Yusufu, Ma Chuang, Yuan Chunxiao
Published 2014-07-08
Cite as Chin J Exp Surg, 2014,31(07): 1431-1433,封3. DOI: 10.3760/cma.j.issn.1001-9030.2014.07.013
Abstract
Objective Explore based on microscopic-CT and three-dimensional printing produce bionic human phalanx scaffold composite adipose-derived stem cells (ADSCs) and recombinant human bone morphogenetic protein 2 (rhBMP-2) of blood vessels in the body,for the clinical treatment of phalangeal defects provide a theoretical basis Methods Take the 3rd generation ADSCs of the rabbits composite rhBMP-2,inoculated with Bionic phalanx bracket mixed culture after 10 days (experimental group) and simply Bionic phalanx bracket (control group) to wrapped in 12 adult beagle dogs lower back on both sides of the pedicle deep fascia,Use self-control,different times gross and histologic staining neovascularization Results The results shows two of the scaffolds in intrafascial both vascular capabilities.The amount of vessels and the expression of ⅧRAg in experimental groups were stronger than those in control groups at 2,4,8,and 16 weeks,the difference was statistically significant (P<0.05).Conclusion The bionic human phalanx scaffold transplant in body with vascular capacity,composite ADSCs-rhBMP-2 can contribute to the degree of vascalarization.
Key words:
Bionic; Three dimensional printing; Adipose stem cells; Recombinant human bone morphogenetic protein-2; Vascularization
Contributor Information
Wang Wei
Department of Microscopic Reconstructive Surgery, the First Affiliated Hospital of Xinjiang Medical University , Urumqi 830000, China
Alimujiang·Abulaiti
Department of Microscopic Reconstructive Surgery, the First Affiliated Hospital of Xinjiang Medical University , Urumqi 830000, China
Aierken·Rehemutula
Department of Microscopic Reconstructive Surgery, the First Affiliated Hospital of Xinjiang Medical University , Urumqi 830000, China
Aihemaitijiang·Yusufu
Department of Microscopic Reconstructive Surgery, the First Affiliated Hospital of Xinjiang Medical University , Urumqi 830000, China
Ma Chuang
Department of Microscopic Reconstructive Surgery, the First Affiliated Hospital of Xinjiang Medical University , Urumqi 830000, China
Yuan Chunxiao
Department of Microscopic Reconstructive Surgery, the First Affiliated Hospital of Xinjiang Medical University , Urumqi 830000, China