The association between interleukin-6 rsl800796 locus single nucleotide polymorphisms in the donors and recipients and concentration/dose ratios of tacrolimus after liver transplantation
Wu Shao-han, Fan Junwei, Chen Dawei, Guo Feng, Zhong Lin, Peng Zhihai
Published 2014-10-08
Cite as Chin J Exp Surg, 2014,31(10): 2138-2141. DOI: 10.3760/cma.j.issn.1001-9030.2014.10.018
Abstract
Objective To investigate the effect of interleukin (IL)-6 rsl800796 locus single nucleotide polymorphisms (SNPs) in the donors and recipients on concentration/dose (C/D) ratios of tacrolimus after liver transplantation.Methods A total of 79 liver transplantation patients whose whole blood concentrations of tacrolimus were determined by using enzyme-linked immunosorbent assay kits were enrolled in the study.Additionally,tacrolimus dosage and trough blood concentration of them was recorded and studied at four weeks after liver transplantation.The SNPs at IL-6 rs1800796 locus were genotyped in both donors and recipients.Then we compared the influence of the different genotypes at this locus on FK506 C/D value for patients.Fluorescent quantitative polymerase chain reaction (FQ-PCR) and immunohistochemical methods were used to test the IL-6 expression levels of mRNA and protein.Results Only donor IL-6 rs1800796 SNPs was associated with tacrolimus C/D ratios at week 2 and 3 after liver transplantation (P < 0.05).While this phenomenon was not finded in recipients from week 1 to 4.The △Ct value in the fast metabolism group contained carriers of genotype CC is (6.6 ± 1.2),while in the slow metabolism group clustered carriers of genotype CG/GG is (7.7 ± 1.1).The difference was significant (P < 0.05).The intermediate expression rate of IL-6 protein in fast metabolism group [80.0% (24/30)] was higher than that in slow metabolism group [48.4% (15/31)].There was significant difference (P < 0.05).Conclusion Donor IL-6 rs1800796 SNPs were closely associated with tacrolimus C/D ratios in the early stage after liver transplantation.
Key words:
Liver transplantation ; Single nucleotide polymorphism ; Tacrolimus ; Interleukin-6
Contributor Information
Wu Shao-han
Department of General Surgery, the First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China
Fan Junwei
Department of General Surgery, the First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China
Chen Dawei
Department of General Surgery, the First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China
Guo Feng
Department of General Surgery, the First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China
Zhong Lin
Department of General Surgery, the First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China
Peng Zhihai
Department of General Surgery, the First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China