Objective To investigate the effect of tumor susceptibility gene 101 (TSG101) on angiogenesis of gastric cancer SGC7901 cells.Methods Cells were divided into the non-transfection group,the empty plasmid transfection group and TSG101 eukaryotic expression plasmid transfection group (the TSG101 transfection group).Then we examined the relationship between TSG101 expression and tumor angiogenesis in vivo and in vitro experiment respectively.To detect the expression of vascular endothelial growth factor (VEGF),vascular endothelial growth factor receptor (VEGFR) and the angiogenesis.Resuits (1) It was showed that the angiogenesis number of HUVEC and the expression levels of VEGF in the TSG101 transfection group was significantly higher than that of the empty plasmid transfection group and the non-transfection group in vitro experiment (19.50 ± 3.02 vs.11.50 ± 2.51,11.34 ± 1.63 and 514.80 ± 18.16 vs.306.58 ± 20.86,305.69 ± 29.93,all P ＜ 0.05).(2) It was showed the microvascular density in tumor tissue of nude mice and the serum VEGF concentration of the TSG101 transfection group was significantly higher than that of the empty plasmid transfection group and the non-transfection group in vivo experiment [11.16±2.31 vs.5.33 ±1.63,4.66 ±1.36 and (340.64±19.15) ng/L vs.(219.34 ± 13.56),(206.10 ± 25.85) ng/L,all P ＜ 0.05].The expressions of VEGF and VEGFR of the TSG101 transfection grou p (0.548 ± 0.032,0.345 ± 0.042) of tumor tissue in nude mice were significantly higher than that of the non-transfection group (0.350 ± 0.034,0.203 ± 0.030) and the empty plasmid transfection group (0.327 ± 0.020,0.206 ± 0.036,P ＜ 0.05).Conclusion The high-expression of TSG101 in SGC7901 cells may promote angiogenesis of gastric cancer by up-regulated expression of VEGF.