Urology
The expression of clusterin in canine renal tubular epithelial cells treated with different concentrations of calcium oxalate
Junjiang Liu, Yunxia Zhang, Shoubin Li, Jinyi Li, Chunhong Yu, Qihong Feng, Gang Wang, Dong Wei, Fuzhen Sun, Tao Yang
Published 2016-06-08
Cite as Chin J Exp Surg, 2016, 33(6): 1488-1489. DOI: 10.3760/cma.j.issn.1001-9030.2016.06.017
Abstract
ObjectiveTo investigate the relationship between clusterin expression and development of renal calculi.
MethodsDetect the expression level of clusterin in canine renal tubular epithelial cells (MDCK) and supernatant fluid after treated with different concentrations of calcium oxalate by Western blotting method and enzyme linked immunosorbent assay (ELISA) method.
ResultsClusterin expressed in both control cells and cells treated with calcium oxalate solution, but obviously stronger in treated cells. The clusterin concentration in supernatant fluid of cells treated with different concentration (300, 500, 900 μg/ml) was (241.33±59.28), (247.67±22.48), (270.67±43.50) pg/ml, respectively. Which was significant higher than that in control, (78.67±39.80) pg/ml (P<0.01); while among groups of different calcium oxalate concentrations treatment, there were no significant difference in clusterin concentration (P>0.05).
ConclusionClusterin expression increased in renal tubular epithelial cells treated with calcium oxalate, which indicate it may be involved in the formation of kidney stones.
Key words:
Kidney stones; Clusterin; Calcium oxalate; Renal tubular epithelial cells
Contributor Information
Junjiang Liu
Department of Urology, Hebei General Hospital, Shijiazhuang 050051, China
Yunxia Zhang
Shoubin Li
Department of Urology, Hebei General Hospital, Shijiazhuang 050051, China
Jinyi Li
Department of Obstetrics and Gynecology, Hebei General Hospital, Shijiazhuang 050051, China (Zhang YX)
Department of Urology, Mount Sinai Hospital, NewYork 91406, America
Chunhong Yu
Department of Ophthalmology, the Kailuan General Hospital, Tangshan 063000, China
Qihong Feng
Department of Ophthalmology, the Kailuan General Hospital, Tangshan 063000, China
Gang Wang
Department of Urology, Hebei General Hospital, Shijiazhuang 050051, China
Dong Wei
Department of Urology, Hebei General Hospital, Shijiazhuang 050051, China
Fuzhen Sun
Department of Urology, Hebei General Hospital, Shijiazhuang 050051, China
Tao Yang
Department of Urology, Hebei General Hospital, Shijiazhuang 050051, China
