Animal Model
One mouse model of intrahepatic cholangiocarcinoma by hydrodynamic injection of sleeping beauty vectors
Fangquan Zhu, Zhongqiang Qin, Hongjun Li, Yong Chen, Yonghai Li, Yi Tan
Published 2017-04-08
Cite as Chin J Exp Surg, 2017, 34(4): 703-705. DOI: 10.3760/cma.j.issn.1001-9030.2017.04.055
Abstract
ObjectiveTo establish one mouse model of intrahepatic cholangiocarcinoma (ICC) for liver cancer reseach.
MethodsThe transposon vectors of sleeping beauty were constructed harboring the activated protein kinase B (myr-Akt) or the activated Notch1 intracellular domain (NICD1), respectively. These vectors were hydrodynamic injected through tail veil. Mice injected with saline was used as the control. Mice were sacrificed around 4 weeks. Tumors were featured by macro-and micro-histological observation. Immunohistological staining and fluorescence staing were used for cytokeratin (CK)19 and Akt cellular expression detected by the sequence Tag of hemagglutinin surface antigen determinant of influenza virus (HA)-tag.
ResultsCompared with the control, mice in the experimental group progressed to tumor with the rate of 100% (14/14). The features of macro-and micro-histological observation indicate the formation of intraheptic cholangiocarcinoma. High percentage (64.3%) of CK19-positive staining in the malignant cells of liver confirmed the tumorigenesis of ICC. Similarly, high rate of HA-positive(42.9%) expression was observed in malignant cells. Colocalization of CK19 and HA staining by immunofluorescence staining also verify high expression.
ConclusionWe provide one method to generate ICC murine model with the advantages of easy-manupilation and time-saving and recapitulation.
Key words:
Intrahepatic cholangiocarcinoma; Hydrodynamic Injection; Sleeping beauty transposons; Mouse, model
Contributor Information
Fangquan Zhu
Department of Hepatobiliary Surgery, the First Affiliated Hospital, Bengbu Medical College, Bengbu 233004, China
Zhongqiang Qin
Department of Hepatobiliary Surgery, the First Affiliated Hospital, Bengbu Medical College, Bengbu 233004, China
Hongjun Li
Laboratory of Pathophysiology, Bengbu Mmedical College, Bengbu 233030, China
Yong Chen
Laboratory of Pathogeny Biology, Bengbu Medical College, Bengbu 233030, China
Yonghai Li
Laboratory of Pathophysiology, Bengbu Mmedical College, Bengbu 233030, China
Yi Tan
Department of Hepatobiliary Surgery, the First Affiliated Hospital, Bengbu Medical College, Bengbu 233004, China
