Experimental Study
Correlation analysis between sperm-associated antigen 9 and epithelial-mesenchymal transition, extracelluar matrix-associated protein in prostate cancer
Chunhua Yang, Meng Ding, Kai Xu, Hang Cao, Ning Liu, Lijun Mao
Published 2017-05-08
Cite as Chin J Exp Surg, 2017, 34(5): 818-820. DOI: 10.3760/cma.j.issn.1001-9030.2017.05.030
Abstract
ObjectiveTo study correlation between sperm-associated antigen 9 (SPAG9) and epithelial-mesenchymal transition (EMT), extracelluar matrix (ECM)-associated protein in prostate cancer.
MethodsThe immunohistochermical analysis was used to detect the expression of SPAG9, E-cadherin, Vimentin and matrix metalloproteinase-2 (MMP-2) in 60 specimens of prostatic carcinoma (PCa) and 30 specimens of benign prostate hyperplasia (BPH). Statistical analyses were used to evaluate the relationship between SPAG9 and E-cadherin, Vimentin, MMP-2.
ResultsThe immunohistochermical analysis showed the average integral absorbance (IA) of each protein in metastatic PCa, nonmetastatic PCa and BPH as follows: for SPAG9 (466.34±13.52, 284.98±25.69 and 106.01±13.58, the differences were all statistically significant, P=0.000); for Vimentin (285.79±15.85, 184.20±21.31 and 79.56±7.96, the differences were all statistically significant, P=0.000); for MMP-2 (269.97±15.74, 175.82±21.81 and 87.86±17.61, the differences were all statistically significant, P=0.000). The IA values of these proteins were reduced successively in the three groups; however, the IA values of E-cadherin (148.62±28.31, 286.73±18.73 and 417.73±12.90, the differences were all statistically significant, P=0.000) were increased successively in the three groups. Pairwise comparisons of each protein’s IA within the three groups revealed the statistically significant differences. There was a positive correlation between SPAG9 and Vimentin (rs=0.443, P=0.014), MMP-2 (rs=0.443, P=0.001) while negative correlation was existed between SPAG9 and E-cadherin (rs=-0.431, P=0.017).
ConclusionSPAG9 may promote the occurrence and development of PCa by regulating EMT, ECM-associated proteins.
Key words:
Sperm-associated antigen 9; Epithelial-mesenchymal transition; Extracelluar matrix; Prostatic carcinoma
Contributor Information
Chunhua Yang
Department of Radiotherapy, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China
Meng Ding
Department of Urology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China
Kai Xu
Department of Urology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China
Hang Cao
Department of Urology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China
Ning Liu
Department of Urology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China
Lijun Mao
Department of Urology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China