Experimental Study
Effect of curcumol on proliferation, apoptosis and enhancer of zeste homolog 2 expression in bladder cancer EJ cells
Li Zhou, Baotong Zhou, Ran Liao, Jiefu Huang, Li Gao, Erdong Wei, Bo Ge
Published 2017-05-08
Cite as Chin J Exp Surg, 2017, 34(5): 815-817. DOI: 10.3760/cma.j.issn.1001-9030.2017.05.029
Abstract
ObjectiveTo observe the effect of curcumol on proliferation, apoptosis and enhancer of zeste homolog 2 (EZH2) expression in human bladder cancer line cell EJ, and to explore its molecular mechanism.
MethodsThe bladder cancer EJ cells were treated with different concentrations of curcumol (12.5, 25.0, 50.0, and 100.0 mg/L) and the control group was given 1% anhydrous ethanol. The inhibitory effect of curcumol on the growth of EJ cells was evaluated by cell counting kit-8 (CCK-8) assay. Special morphological changes of apoptosis were observed by Hoechst 33258 flourescence staning. Flow cytometry technique was used to detect the apoptosis rate of each experimental group. The expression of EZH2 mRNA and protein was examined with reverse transcriptase-polymerase chain reaction (RT-PCR) and Western boltting.
ResultsEJ cells proliferation was obviously inhibited by curcumol in a time- and concentration-dependent manner. The inhibiton rate was (32.94±0.93)%, (46.75±1.28)%, (58.16±0.76)% and (69.13±1.94)%, and the apoptosis rate was (3.60±0.46)%, (6.70±1.83)%, (16.20±1.61)% and (28.70±2.65)% in curcumol-treated (12.5, 25.0, 50.0, and 100.0 mg/L) groups respectively. A typical form of apoptosis was detected in the high concentration of curcumol by fluorescent inverted phase contrast microscope. As compared with the control group, with the increases in concentrations of curcumenol, the expression of EZH2 mRNA and protein was gradually declined.
ConclusionCurcumol could significantly inhibit the growth of EJ cells and induce the apoptosis, which may be related to the down-regulation of EZH2.
Key words:
Curcumol; Bladder cancer; Enhancer of zeste homolog 2
Contributor Information
Li Zhou
Department of Urology, the Affiliated Hospital of Guilin Medical University, Guilin 541001, China
Baotong Zhou
Department of Urology, the Affiliated Hospital of Guilin Medical University, Guilin 541001, China
Ran Liao
Department of Urology, the Second Affiliated Hospital of Chengdu Medical College, Chengdu 610051, China
Jiefu Huang
Department of Urology, the Affiliated Hospital of Guilin Medical University, Guilin 541001, China
Li Gao
Department of Urology, the Affiliated Hospital of Guilin Medical University, Guilin 541001, China
Erdong Wei
Department of Urology, the Affiliated Hospital of Guilin Medical University, Guilin 541001, China
Bo Ge
Department of Urology, the Affiliated Hospital of Guilin Medical University, Guilin 541001, China