Experimental Study
Paired related homoeobox 1 promotes the migration ability of breast cancer cells by inducing epithelial-mesenchymal transition
Wenjuan Wang, Zhidong Lyu, Lin Zhang, Zhen Song, Na Kang, Qiaoyu Chen, Funian Li
Published 2017-06-08
Cite as Chin J Exp Surg, 2017, 34(6): 959-962. DOI: 10.3760/cma.j.issn.1001-9030.2017.06.018
Abstract
ObjectiveTo investigate the effect of paired related homoeobox 1 (Prrx1) on the epithelial-mesenchymal transition (EMT) and migration ability of breast cancer cells.
MethodsBreast cancer cell lines (including BT-549, MDA-MB-468 and MCF-7) were screened by real-time quantitative polymerase chain reaction (Real-time PCR) to obtain cells with the lowest expression of Prrx1. Then the cells were transfected by Prrx1 over-expression letiviral vectors. The morphologic changes of the breast cancer cells were observed after the transfection was verified to be accomplished through Western blotting. At last, the influence of Prrx1 on the migration ability of the breast cancer cells was detected by Transwell assay.
ResultsThe exprossioris of Prrx1 mRNA in MCF-7, MDA-MB-468, BT-549 cells are 15.05±0.18, 11.78±0.42, 7.98±0.37. Thus, MCF-7 cells were screened to be the lowest Prrx1 expression cells in the three kinds of breast cancer cells. The MCF-7 cells were transfected by Prrx1 over-expressing letiviral vectors. It was found that the morphology of these cells changed from polygon to long spindle appearance. The Real-time PCR results showed that the exprossioris of Prrx1 mRNA in experimental, control and blank control groups were 2.16±0.07, 10.18±0.07 and 10.2±0.07, those of E-adherin mRNA were 4.00±0.02, 3.56±0.02 and 3.51±0.05, and those of Vimentin mRNA were 8.79±0.42, 10.18±0.06 and 9.97±0.05, respectively. The mRNA levels of Prrx1, E-adherin and vimentin were 259.57, 0.71, and 2.27 times compared with groups without transfection respectively (t=227.100, P=0.000; t=6.007, P=0.000; t=11.900, P=0.000), while the difference between the control group and the lentiviral vectors group was not statistically significant (t=1.315, P=0.202; t=0.730, P=0.476; t=2.052, P=0.057). Transwell assay showed that the number of the migrating cells in over-expression group (73.40±10.21) was greater than in both non-treated group (45.60±6.62) and lentiviral vectors group (44.60±8.24; t=6.837, P=0.000), while the difference between the control group and the lentiviral vectors group was not statistically significant (t=0.295, P=0.772).
ConclusionPrrx1 over-expression can promote EMT of breast cancer cells and improve their migration ability.
Key words:
Breast cancer; Epithelial-mesenchymal transition; Paired related homoeobox1; Migration
Contributor Information
Wenjuan Wang
Clinical Medicine of Medical College of Qingdao University, Qingdao 266000, China
Zhidong Lyu
Breast Disease Diagnosis and Treatment Centre, Affiliated Hospital of Qingdao University, Qingdao 266000, China
Lin Zhang
Department of Surgery, Affiliated Tai’an Central Hospital, Tai’an 271000, China
Zhen Song
Breast Disease Diagnosis and Treatment Centre, Affiliated Hospital of Qingdao University, Qingdao 266000, China
Na Kang
Operating Room, Affiliated Hospital of Qingdao University, Qingdao 266000, China
Qiaoyu Chen
Breast Disease Diagnosis and Treatment Centre, Affiliated Hospital of Qingdao University, Qingdao 266000, China
Funian Li
Breast Disease Diagnosis and Treatment Centre, Affiliated Hospital of Qingdao University, Qingdao 266000, China
