Orthopeadic Surgery
Effect of deferoxamine on osteoblast differentiation under iron overload environment
Peng Zhang, Sheng Wang, Ye Yuan, Bingchen Shan, Liang Wang, Fan Yang, Peng Jia, Youjia Xu
Published 2018-01-08
Cite as Chin J Exp Surg, 2018, 35(1): 8-10. DOI: 10.3760/cma.j.issn.1001-9030.2018.01.004
Abstract
ObjectiveTo study the influence of eferoxamine (DFO) intervention on osteoblast defferentiation under iron overload environment [using ferric ammonium citrate (FAC) to offer iron overload environment].
MethodsThe FAC and DFO intervention dosage was determined by method of cell counting kit-8 (CCK-8). Experiment was divided into three groups: control group, 50 μmol/L FAC intervention group, and FAC + DFO group (50 μmol/L FAC intervention + 20 μmol/L DFO). After intervention, alkaline phosphatase staining and quantitation, alizarin red staining and calcium nodule count were carried out. Real-time quantitative polymerase chain reaction detecting system (QPCR) was used to detect the osteogenesis related gene [runt related transcription factor 2 (Runx2), special protein 7 (Sp7), bone gamma-carboxyglutamic acid-containing protein (Bglap), osteoprotegerin (OPG)] mRNA expression level.
ResultsCCK-8 showed 50 μmol/L FAC [(95.6±0.7)%], or 20 μmol/L DFO [(97.6±0.5)%] did not influence the cell viability, with the difference being not statistical differences among groups (P=0.427). As compared with control group, the expression of osteogenesis related genes (Runx2, Sp7, Bglap and OPG) was significantly decreased in the FAC group. As compared with the FAC group, the expression of osteogenesis related genes was significantly increased in the FAC + DFO group (P=0.026). As compared with the control group, the activity of alkaline phosphatase (ALP) staining and quantitation, alizarin red staining and nodule count were decreased in the FAC group. As compared with the FAC group (36±3), the nodule count in the FAC + DFO group (120±6) was increased significantly (P=0.017).
ConclusionOsteoblast differentiation is restrained under the iron overload environment. DFO intervention can restore osteoblast differentiation to a certain extent under the iron overload environment.
Key words:
Deferoxamine; Osteoblast; Iron overload environment; Osteoporosis
Contributor Information
Peng Zhang
Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Soochow 215004, China
Sheng Wang
Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Soochow 215004, China
Ye Yuan
Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Soochow 215004, China
Bingchen Shan
Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Soochow 215004, China
Liang Wang
Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Soochow 215004, China
Fan Yang
Osteoporosis Institute of Soochow University, Suzhou 215004
Peng Jia
Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Soochow 215004, China
Youjia Xu
Department of Orthopedics, the Second Affiliated Hospital of Soochow University, Soochow 215004, China