To investigate the inhibitory effect of CXC chemokine receptor 4 (CXCR4) short hairpin RNA (shRNA) mediated by recombinant adenovirus vector on tumor growth in nude mice with non small cell lung cancer.

Inhibiting the expression of CXCR4 in non-small cell lung cancer cells A549 using CXCR4 shRNA mediated by recombinant adenovirus vector. The expression of CXCR4 mRNA in A549 cells were detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) method. Two groups of nude mice tumor model with non small cell lung cancer were established: the CXCR4 mRNA group and the empty virus group. The tumor volume, quality, quantity and survival time were recorded. The survival rate and tumor inhibition rate of nude mice in two groups were calculated. The expression levels of vascular endothelial growth factor (VEGF) protein in tumor tissue of mice after death was detected by Westem blotting method.

Compared with the empty virus group (1.17±0.19), the expression level of CXCR4 mRNA in A549 cells infected by recombinant adenovirus vector (0.26±0.10) significantly decreased (P=0.000). The average volume, mean mass and average number of tumor in group CXCR4-shRNA were significantly lower than that of the nude virus group, (P=0.000). The CXCR4-shRNA group the average survival time was significantly longer than the empty virus group (P=0.000); CXCR4-shRNA group survival rate and delayed tumor growth inhibition rate was significantly higher than that of the empty virus group (P=0.000), CXCR4-shRNA group, VEGF protein expression level (0.28±0.05) was significantly lower than that of the empty virus group (1.02±0.23), the difference was statistically significant (P=0.000).

CXCR4 shRNA mediated by recombinant adenovirus vector can inhibit the growth and metastasis in nude mice with non small cell lung cancer.

CXC chemokine receptor 4 short hairpin RNA; Non small cell lung cancer; Nude mice; Tumor inhibition