Pancreatic Surgery
Effect of Aurora-A inhibitor 1 on proliferation, apoptosis of PANC-1 cells
Zeling Cai, Sheng Wang, Yonghuan Mao, Jin Wang, Kai Wu, Jia Zhou, Xiagang Luo, Chunzhao Yu
Published 2018-08-08
Cite as Chin J Exp Surg, 2018, 35(8): 1403-1405. DOI: 10.3760/cma.j.issn.1001-9030.2018.08.004
Abstract
ObjectiveTo investigate the expression of Aurora kinase A in normal human pancreatic epithelial cell and pancreatic cancer cell lines, and explore the role of Aurora-A inhibitor 1 on proliferation, apoptosis of pancreatic cancer cell PANC-1.
MethodsThe expression level of Aurora-A and p-Aurora-A protein in two different cells was detected using Western blotting. Cell counting kit-8 (CCK-8) was constructed to investigate cell proliferation. Flow cytometry (FCM) and Western blotting were used to detect cell cycle and cell apoptosis.
ResultsWestern blotting indicated that Aurora-A and p-Aurora-A were over-expression in pancreatic cancer. The results of CCK-8 showed that Aurora-A could inhibit the proliferation of PANC-1 (P=0.019). The test of FCM showed that Aurora-A inhibitor arrest the cells during G2/M [Blank: (9.6±2.3)%, dimethyl sulfoxide (DMSO): (7.5±4.8)%, Aurora-A inhibitor 1: (61.2±4.0)%] and Western blotting further showed p21 was up-regulated (Blank: 1.000±0.000, DMSO: 1.671±0.111, Aurora-A inhibitor 1: 3.208±0.284). Moreover, FCM indicated that the apoptosis was significantly increased in Aurora-A inhibitor 1 group after treatment (Blank: 1.000±0.000, DMSO: 1.053±0.108, Aurora-A inhibitor 1: 1.902±0.276). The results of Western blotting showed the expression of Cleavage of cysteinyl aspartate specific protease (Cleaved Caspase-3) was raised (Blank: 1.000±0.000, DMSO: 1.274±0.112, Aurora-A inhibitor 1: 1.754±0.062).
ConclusionAurora-A and p-Aurora-A is over-expression in pancreatic cancer. Inhibition of p-Aurora-A by Aurora-A inhibitor 1 can arrest pancreatic cancer cell in G2/M. Moreover, Aurora-A inhibitor 1 decrease proliferation and promote cell apoptosis.
Key words:
Pancreatic cancer; Aurora-A; Inhibitors; Proliferation; Apoptosis
Contributor Information
Zeling Cai
Department of General Surgery, the Second Clinical Medical School of Nanjing Medical University, Nanjing 210011, China
Sheng Wang
Department of General Surgery, the Second Clinical Medical School of Nanjing Medical University, Nanjing 210011, China
Yonghuan Mao
Department of General Surgery, the Second Clinical Medical School of Nanjing Medical University, Nanjing 210011, China
Jin Wang
Department of Neurosurgery, the First Affiliated Hospitol of Nanjing Medical University, Nanjing 210029, China
Kai Wu
Department of General Surgery, the Second Clinical Medical School of Nanjing Medical University, Nanjing 210011, China
Jia Zhou
Department of General Surgery, the Second Clinical Medical School of Nanjing Medical University, Nanjing 210011, China
Xiagang Luo
Department of General Surgery, the Second Clinical Medical School of Nanjing Medical University, Nanjing 210011, China
Chunzhao Yu
Department of General Surgery, the Second Clinical Medical School of Nanjing Medical University, Nanjing 210011, China
