Animal Model
The establishment and evaluation of microvascular visualization model in bone tissue
Yajie Lu, Minghui Li, Zuoyao Long, Haoqiang Zhang, Xin Xiao, Jie Wu, Zhen Wang
Published 2018-12-08
Cite as Chin J Exp Surg, 2018, 35(12): 2336-2338. DOI: 10.3760/cma.j.issn.1001-9030.2018.12.053
Abstract
ObjectiveTo explore a new method for visualization and morphologic study of intraosseous microvessels, measure the main parameters of the vesselsand evaluate the reliability of this model.
MethodsAbdominal aorta microfill perfusion in 10 New Zealand white rabbits were used. The specimens of the lower femur were fixed and decalcified sequentially, then the blood vessels were analyzed by microCT scan and three-dimensional reconstruction. The microvessels distribution and morphology of the bone were observed under the background of fluorescence by making the specimen into slices with a thickness of 300 μm. The main parameters of blood vessels were measured and evaluated.
ResultsThe structures of the intraosseous microvessels was clearly reconstructed in three dimensions by decalcification which can reduce the interference of the CT value of bone tissue to the CT value of the perfusion fluid. The distribution and morphology of the microvessels in the bone tissue were well displayed in the thick tissue slices in the fluorescence background imaging. There was no statistical difference in the number of blood vessels, mean vessel diameter and vascular percentage of the unit area between the results of microCT and histology (P=0.075, 0.921, 0.661). In histological Histology, the visual minimum blood vessel diameter reached the level of capillaries, significantly less than the results of microCT [(9.03±1.14) μm vs. (12.61±2.01) μm, P=0.038].
ConclusionMicroCT scan three-dimensional reconstruction combined with thick slice fluorescence background imaging can be used as a reliable method for the visualization and morphological study of intraosseous microvessels.
Key words:
Bone; Microvessel; Visualization; Histology; MicroCT
Contributor Information
Yajie Lu
Department of Orthopaedics, Xijing Hospital, the Air Force MilitaryMedical University, Xi’an 710032, China
Minghui Li
Department of Orthopaedics, Xijing Hospital, the Air Force MilitaryMedical University, Xi’an 710032, China
Zuoyao Long
Department of Orthopaedics, Xijing Hospital, the Air Force MilitaryMedical University, Xi’an 710032, China
Haoqiang Zhang
Department of Orthopaedics, Lanzhou General Hospital of the People’s Liberation Army, Lanzhou 730050, China
Xin Xiao
Department of Orthopaedics, Xijing Hospital, the Air Force MilitaryMedical University, Xi’an 710032, China
Jie Wu
Department of Orthopaedics, Xijing Hospital, the Air Force MilitaryMedical University, Xi’an 710032, China
Zhen Wang
Department of Orthopaedics, Xijing Hospital, the Air Force MilitaryMedical University, Xi’an 710032, China