Urology
Overexpressed deoxycytidine triphosphate pyrophosphatase 1 promotes cell-cycle in LNCaP
Ye Yongkang, Mi Qiwu, Zeng Yanru, Lu Jianming, Zhong Weide
Published 2018-12-08
Cite as Chin J Exp Surg, 2018,35(12): 2190-2192. DOI: 10.3760/cma.j.issn.1001-9030.2018.12.002
Abstract
ObjectiveTo investigate the expression of human deoxycytidine triphosphate pyrophosphatase 1 (dCTP pyrophosphatase 1) in prostate cancer tissues and LNCaP cells, and observe the effect of high expression of dCTP pyrophosphatase 1 on LNCaP cell cycle.
MethodsThe expression of dCTP pyrophosphatase 1 in prostate cancer and non-cancer tissue was analyzed by immunohistochemistry. The expression of dCTP pyrophosphatase 1 protein in LNCaP and RWPE-1 cells was detected by Western blotting (LNCaP: 1.26±0.13 vs. RWPE-1: 0.52±0.01, P=0.000). The dCTP pyrophosphatase 1 overexpressed LNCaP cell lines were constructed and cell cycle changes were detected by flow cytometry (LNCaP-dCTP pyrophosphatase 1: 48.23±0.66 vs. LNCaP-NC: 30.10±1.01, P=0.022).
ResultsThe expression of dCTP pyrophosphatase 1 in cancer tissues was up-regulated (IHC score: non-cancer: 2.00±1.55, cancer: 4.00±1.52, P=0.000), and the high expression of dCTP pyrophosphatase 1 increased the S+ G2 phase of LNCaP cells (P=0.000).
ConclusionHigh expression of dCTP pyrophosphatase 1 in prostate cancer promotes the division of prostate cancer cells.
Key words:
Deoxycytidine triphosphate pyrophosphatase 1; Prostate cancer; Cell cycle
Contributor Information
Ye Yongkang
Department of Urology, Dongguan People’s Hospital Affiliated to Southern Medical University, Dongguan 523059, China
Mi Qiwu
Department of Urology, Dongguan People’s Hospital Affiliated to Southern Medical University, Dongguan 523059, China
Zeng Yanru
Department of Urology, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Key Laboratory of Clinical Molecular Medicine and Molecular Diagnosis, Guangzhou 510180, China
Lu Jianming
Department of Urology, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Key Laboratory of Clinical Molecular Medicine and Molecular Diagnosis, Guangzhou 510180, China
Zhong Weide
Department of Urology, Guangzhou First People’s Hospital, the Second Affiliated Hospital of South China University of Technology, Key Laboratory of Clinical Molecular Medicine and Molecular Diagnosis, Guangzhou 510180, China
