Orthopeadic Surgery
Differential expression of Gab2 and BMI 1 in primary osteosarcoma and matched lung metastasis and its clinical significance
Jiazhen Li, Yongliang Yang, Yan Zhang, Xinchang Lu, Yi Zhang, Yongkui Liu, Zhenhua Bai
Published 2019-01-08
Cite as Chin J Exp Surg, 2019, 36(1): 47-50. DOI: 10.3760/cma.j.issn.1001-9030.2019.01.015
Abstract
ObjectiveTo investigate the significance of differential expression of Gab2 and BMI 1 in primary lesions of osteosarcoma and corresponding lung metastasis specimens.
MethodsWe collected 24 patients with osteosarcoma in our hospital. All patients underwent neoadjuvant chemotherapy after definite diagnosis. The specimens of 24 patients with osteosarcoma were selected as the primary group and their corresponding pulmonary metastases. The specimens were used as the metastasis group. The primary lesions of 32 patients with osteosarcoma who did not metastasize were selected as the control group. The immunohistochemical method was used to detect Gab2 protein, BMI 1 protein and proliferation cell nuclear antigen (Ki-67) protein in the primary group and lung metastasis group. The expression of the control group was positive (+ ) with a positive cell ratio greater than 25%, 5% to 25% was weakly positive (+ ), and less than 5% was negative (-).
ResultsThe positive rate of Gab2 in the primary group (37.5%) was significantly lower than that in the metastatic group (75%), and higher than that in the control group (16.7%). The difference was statistically significant (P<0.05). The positive rate of BMI 1 gene in the primary group (37.5%) was higher than that in the control group (12.5%), which was statistically significant (P<0.05), but different from the metastatic group (positive rate 33.3%). There was no statistical significance (P>0.05); the positive rate of Ki-67 in the primary group (45.8%) was significantly lower than that in the metastatic group (79.2%), which was also higher than that in the control group (25%). The differences were statistically significant (P<0.05). In addition, the Gab2 gene was positively correlated with Ki-67 (r=0.752, P<0.01), but negatively correlated with BMI-1 expression (r=-0.655, P<0.05).
ConclusionThe high expression of Gab2 gene is associated with lung metastasis of osteosarcoma and negatively correlated with the expression of BMI 1 gene, However, high expression of the BMI 1 gene is also associated with lung metastasis in osteosarcoma.
Key words:
Osteosarcoma lung metastasis; Gab2; BMI 1; Paired specimen; immunochemistry
Contributor Information
Jiazhen Li
Department of Orthopaedics, the First Affilated Hospital of Zhengzhou University, Zhengzhou 450052
Yongliang Yang
Yan Zhang
Xinchang Lu
Yi Zhang
Yongkui Liu
Zhenhua Bai