Pancreatic Surgery
The effect of salt-induced kinase 1 on proliferation, migration and invasion of human pancreatic cell lines HPAC, BxPC3 and PANC-1
Meng Li, De He, Guangnian Ma, Zhijian Li, Siyuan Deng
Published 2019-04-08
Cite as Chin J Exp Surg, 2019, 36(4): 612-614. DOI: 10.3760/cma.j.issn.1001-9030.2019.04.006
Abstract
ObjectiveTo investigate the effect of salt-induced kinase 1 (SIK1) on the proliferation, migration and invasion of pancreatic cells.
MethodsSIK1-mimic was transfected into HPAC, BxPC3 and PANC-1 cell lines. The expression of SIK1 was detected by real-time quantitative polymerase chain reaction (Real-time PCR) after 48 h. Cell proliferation was measured by cell counting kit-8 (CCK-8). The migration ability was assayed by would healing test. Transwell invasion assay was used to measure cell invasion
ResultsThe expression of SIK1 in HPAC, BxPC3 and PANC-1 cells was significantly lower than that in normal pancreatic cells (0.16±0.06, 0.16±0.03, 0.19±0.03, P<0.01). The expression of SIK1 in HPAC, BxPC3 and PANC-1 cells was significantly higher (3.66±0.49, 1.91±0.35 and 2.04±0.19, P<0.01) after transfection with SIK1 mimic. CCK-8 results showed that the proliferation of HPAC, BxPC3 and PANC-1 cells was significantly reduced (0.67±0.02 vs. 0.61±0.03, P<0.01; 0.79±0.03 vs. 0.70±0.02, P<0.01; 0.87±0.02 vs. 0.75±0.03, P<0.01). The results of wound healing test showed that the migration of HPAC, BxPC3 and PANC-1 cells was inhibited [(169.82±7.76) vs. (106.89±7.79) μm, P<0.01; (106.95±6.29) vs. (62.79±9.55) μm, P<0.01; (105.38±7.54) vs. (82.41±10.74) μm, tPACN-1=4.287, P<0.01]. Transwell cell invasion assay showed significant inhibition of HPAC, BxPC3 and PANC-1 cell invasion [(63.50±3.51) vs. (47.83±3.19), P<0.01; (34.50±3.94) vs. (21.50±2.51), P<0.01; (52.50±2.43) vs. (32.83±2.14), P<0.01].
ConclusionOverexpression of SIK1 inhibited the proliferation, migration and invasion of HPAC, BxPC3 and PANC-1 cell lines.
Key words:
Pancreatic carcinoma; Salt-inducible kinase 1; Proliferation; Migration; Invasion
Contributor Information
Meng Li
Department of General Surgery, Affiliated Baoan Hospital of Southern Medical University, Shenzhen 518101, China
Graduate School, Guangdong Medical University, Zhanjiang 524023, China
De He
Department of General Surgery, Affiliated Baoan Hospital of Southern Medical University, Shenzhen 518101, China
Guangnian Ma
Department of General Surgery, Affiliated Baoan Hospital of Southern Medical University, Shenzhen 518101, China
Zhijian Li
Graduate School, Guangdong Medical University, Zhanjiang 524023, China
Siyuan Deng
Graduate School, Guangdong Medical University, Zhanjiang 524023, China
