Neurosurgery
Bone marrow mesenchymal stem cells intro-ventricular transplantation combined with intraperitoneal injection of ginkgolide K for focal cerebral ischemia/reperfusion injury in rats
Huaiyong Hao, Minmin He, Yongtao Li, Chao Wu, Hongxin Ma, Zenghua Wei, Baosheng Huang
Published 2019-08-08
Cite as Chin J Exp Surg, 2019, 36(8): 1377-1380. DOI: 10.3760/cma.j.issn.1001-9030.2019.08.009
Abstract
ObjectiveTo investigate the neuroprotective effect of combined application of bone marrow mesenchymal stem cells (BMSCs) and Ginkgolide K (GK) intraperitoneal injection for rat focal cerebral ischemia.
MethodsIn all rats, the middle cerebral artery occlusion (MCAO) was produced by occlusion of right internal carotid artery with a nylon monofilament. Fifty male Sprague-Dawley (SD) rats were divided into five groups by the random digits table, 10 rats in each group, including Sham group, saline solution intraperitoneal injection group, GK intraperitoneal injection group, BMSCs+ saline solution intraperitoneal group, and BMSCs+ GK intraperitoneal injection group. The neurological defective scores were assessed at 72, 120, 168 h after reperfusion respectively in each experiment and all the animals were then decapitated to determine the brain infarct volume and brain water content after 168 h. The expression of vascular endothelial growth factor (VEGF) was detected by Western blotting.
ResultsThe expression of VEGF was increased significantly after intraperitoneal injection of GK. Combined treatment of BMSCs and GK could significantly decrease the number of apoptosis cells [(18.8±3.6)/HP, F=167.531, P<0.01] and infarct volumes [(17.31±5.24)%, F=34.910, P<0.01], brain water content in ischemia hemisphere [(73.55±0.53)%, F=65.128, P<0.01]. The values of neurological defective scores in BMSCs+ GK injection group were significantly lower than the rest groups [120 h: (3.51±0.46), F=15.473, P<0.01; 168 h: (2.03±0.35), F=21.711, P<0.01].
ConclusionCombined treatment of BMSCs intro-ventricular transplantation and GK intraperitoneal injection produces a synergistic neuroprotective effect.
Key words:
Vascular endothelial growth factor; Bone marrow; Mesenchymal stem cells; Ginkgolide K
Contributor Information
Huaiyong Hao
Department of Neurosurgery, Tengzhou Central People’s Hospital Affiliated to Jining Medical College, Tengzhou 277599, China
Minmin He
Department of Paediatrics, Tengzhou Central People’s Hospital Affiliated to Jining Medical College, Tengzhou 277599, China
Yongtao Li
Department of Neurosurgery, Tengzhou Central People’s Hospital Affiliated to Jining Medical College, Tengzhou 277599, China
Chao Wu
Department of Neurosurgery, Tengzhou Central People’s Hospital Affiliated to Jining Medical College, Tengzhou 277599, China
Hongxin Ma
Department of Neurosurgery, Tengzhou Central People’s Hospital Affiliated to Jining Medical College, Tengzhou 277599, China
Zenghua Wei
Department of Neurosurgery, Tengzhou Central People’s Hospital Affiliated to Jining Medical College, Tengzhou 277599, China
Baosheng Huang
Department of Neurosurgery, Sir Run Run Hospital, Nanjing Medical University, Nanjing 210006, China