Cardio-thoracic Surgery
Impact and mechanism of PMs-3 to epithelial mesenchymal transformation of non-small cell lung cancer cell line A549
Zihao Chen, Xian Li, Jing Jia, Ping Li, Yinghong Jiang, Hongping Ju
Published 2019-09-08
Cite as Chin J Exp Surg, 2019, 36(9): 1536-1539. DOI: 10.3760/cma.j.issn.1001-9030.2019.09.003
Abstract
ObjectiveTo explore Impact and mechanism of PMs-3 to epithelial mesenchymal transformation (EMT) of non-small cell lung cancer (NSCLC) cell line A549.
MethodsNanocomposite structure synthetized by our team was utilized on human NSCLC cell line A549 and human normal pulmonary epithelial cell line BEAS-2B with concentrations of 0, 20, 40, 60, 80, 100, 120 nmol/L for 48 h. Methyl thiazol tetrazolium (MTT) assay was employed to detect the effect of PMs-3 on A549 cells. Wound assay and Transwell chamber assay were used to test the migration and invasion of PMs-3 on A549 cells. EMT related genes were detected with real time PCR (RT-qPCR) and Western blotting.
ResultsResults of MTT assay detected that PMs-3 could inhibit activity of A549 cells significantly, and inhibition rate was (3.203±1.222)% (0 nmol/L), (11.958±4.000)% (20 nmol/L), (25.348±7.710)% (40 nmol/L), (34.775±9.155)% (60 nmol/L), (41.163±10.988)% (80 nmol/L), (50.430±9.743)% (100 nmol/L), (51.895±6.161)% (120 nmol/L), which showed a dose-dependent manner (F=35.451, P<0.01). Inhibition rate of BEAS-2B cells treated with PMs-3 (100 nmol/L) was (24.103±5.755)%, which was lower than that of A549 cells (t=5.699, P<0.01). Migrationrate of A549 cells in PMs-3 group was (34.545±7.888)%, which was lower than in control group (77.408±5.442) (t=-10.956, P<0.01). Cells through the Transwell chamber in PMs-3 group was 125.333±25.137), which was less than in control group (200.667±19.967) (t=-5.748, P<0.01). mRNA and proteins of EMT related genes varied after PMs-3 treated as following: expression of E-cadherin increased significantly in PMs-3 group compared with control group, while expression of N-cadherin, Snail decreased significantly in PMs-3 group compared with control group (t=-5.753, -10.538, 5.583, 5.771, 6.660, 4.120, P<0.05); no significant variation of Vimentin was verified between PMs-3 group and control group (t=-0.323, 1.179, P>0.05).
ConclusionPMs-3 could inhibit NSCLC cells via regulating EMT.
Key words:
Non-small cell lung cancer; PMs-3; Epithelial mesenchymal transformation; Drug treatment
Contributor Information
Zihao Chen
Graduate School of Hebei Medical University, Shijiazhuang 050011, China
Xian Li
School of Medicine, Kunming University, Kunming 650214, China
Jing Jia
School of Medicine, Kunming University, Kunming 650214, China
Ping Li
School of Medicine, Kunming University, Kunming 650214, China
Yinghong Jiang
School of Medicine, Kunming University, Kunming 650214, China
Hongping Ju
School of Medicine, Kunming University, Kunming 650214, China
