Experimental Study
Effects of non-coding RNA00460 targeting microRNA-539/SRY-related high mobility group-box gene 4 axis on proliferation and migration of glioma cells
Chang Bo, Gao Fei, Liang Qinghua
Published 2019-10-08
Cite as Chin J Exp Surg, 2019,36(10): 1802-1805. DOI: 10.3760/cma.j.issn.1001-9030.2019.10.021
Abstract
ObjectiveTo investigate the effects of non-coding RNA00460 targeting microRNA (miRNA, miR)-539-SRY-related high mobility group-box gene 4 (SOX4) on the proliferation and migration of glioma cells.
MethodsTwenty cases of normal brain glia and 20 cases of glioma were collected. The expression levels of lncRNA 00460 and miR-539 were analyzed by fluorescence quantitative polymerase chain reaction (PCR). Bioinformatics was used to analyze the relationship between lncRNA 00460 and miR-539 and target genes; knockdown cell line lncRNA 00460 and miR-539 overexpression cell line were constructed in U251 glioma cell line. cell counting kit-8 (CCK-8) and Transwell were used to analyze the proliferation and migration ability of glioma cells treated with different methods; xenograft tumor model was used to analyze the proliferation ability of glioma cells in vivo.
ResultsCompared with normal brain glioma cells [(1.21±0.25), (1.35±0.29)], the expression of lncRNA 00460 (3.41±0.51) was significantly increased in glioma tissues, while the expression of microR-539 (0.39±0.11) was significantly decreased (t=3.189, 4.208, P<0.05). Bioinformatics studies have found that lncRNA 00460 regulates the expression of miR-539 and further regulates the expression of SOX4 protein. The knockdown of lncRNA 00460 significantly inhibited the proliferation and migration of glioma cells (F=3.729, 3.109, P<0.05). Overexpression of miR-539 significantly increased the proliferation and migration of glioma cells (F=5.129, 5.114, P<0.05). In vivo xenogeneic tumorigenesis model showed that knockdown of lncRNA 00460 significantly inhibited the growth of glioma cells in vivo, while over-expression of microRNA539 significantly promoted the proliferation of glioma cells (F=5.194, 3.104, P<0.05).
ConclusionlncRNA 00460 regulates the proliferation and migration of glioma cells by targeting the miR-539/SOX4 axis.
Key words:
Long non-coding RNA 00460; MicroRNA-539; Glioma cells; Proliferation; Migration
Contributor Information
Chang Bo
Department of Neurosurgery, Anyang People’s Hospital, Anyang 455000, China
Gao Fei
Department of Ophthalmology, Anyang People’s Hospital, Anyang 455000, China
Liang Qinghua
Department of Neurosurgery, Henan People’s Hospital, Zhengzhou 450000, China