Liver Surgery
Complement factor CD59 deficiency significantly aggravates cholestasis liver injury in mice
Lan Chaozhi, Guo Zhenya, Yu Xibing, Chen Yubing, Yu Shuiping, Yuan Guandou, He Songqing, Lyu Jun
Published 2019-10-08
Cite as Chin J Exp Surg, 2019,36(10): 1774-1776. DOI: 10.3760/cma.j.issn.1001-9030.2019.10.013
Abstract
ObjectiveTo investigate the role of complement factor CD59 in cholestatic liver injury in mouse bile duct ligation (BDL) model.
MethodsThe cholestasis model of mice was induced by BDL. The relevant indexes were detected 3 days after operation.
ResultsIncreased alanine aminotransferase [ALT: (730.67±104.50) U/L vs. (311.33±52.47) U/L, t=8.784, P<0.01], aspartate aminotransferase [AST: (877.17±76.31) U/L vs. (454.83±45.87) U/L, t=11.620, P<0.01], and total bilirubin [TBIL: (244.15±32.22) U/L vs. (141.85±41.51) U/L, t=4.769, P<0.01] were observed in CD59 deficient animals with BDL when compared with wildtype control, indicating that CD59 deficiency significantly aggravates cholestatic liver injury in mice. However, there was no significant difference in C3a [(408.30±167.82) μg/L vs. (269.48±49.33) μg/L, t=1.587, P>0.05] and C5a [(284.43±80.88) μg/L vs. (212.95±13.53) μg/L, t=1.743, P>0.05] levels between the two BDL groups, suggesting that CD59 deficiency did not impact in vivo generation of C3 and C5 activation products. An increased membrane attack complex (MAC) deposition in CD59 deficiency animals could be monitored.
ConclusionThe present study proves a functional role of CD59 during cholestasis. CD59 deficiency leads to aggravated cholestatic liver injury by up-regulating the expression of MAC. CD59 appears to be an attractive target for future therapeutic intervention in chronic cholestatic liver disease.
Key words:
Cholestatic liver injury; CD59; Complement; Bile duct ligation; Membrane attack complex
Contributor Information
Lan Chaozhi
Department of Hepatobiliary Surgery, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China
Guo Zhenya
Yu Xibing
Chen Yubing
Yu Shuiping
Yuan Guandou
He Songqing
Lyu Jun