To study the effects of Actinidia root extract on proliferation, apoptosis, migration and invasion of Hepatocellular carcinoma cells.

The microRNA (miRNA, miR)-NC group (transfected miR-NC), the miR-34-5p group (transfected miR-34-5p mimics), the RAE + anti-miR-NC group (transfected anti-miR-NC), RAE + anti-miR-34-5p group (transfected anti-miR-34-5p), all were transfected into HCCLM3 cells by liposome method; the expression of miR-34-5p was detected by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR); the protein expression of B cell lymphoma/leukemia-2 (bcl-2), bcl-2 associated X protein (bax), E-cadherin and membrane palm glycosylated protein-2 (MPP-2) were detected by Western blotting; flow cytometry to detect apoptosis; transwell chamber to detect cell migration and invasion.

Compared with Con group, Actinidia root extract (RAE) (1, 10, 100 mg/L) significantly inhibited the proliferation of hepatoma cells in a concentration-dependent manner (F=421.057, 625.513, 676.701, 578.243, 880.710 and 206.608, P<0.05), and the optimal concentration was 10 mg/L. It can inhibit the proliferation, migration and invasion of hepatocarcinoma cells, promote apoptosis, down-regulate bcl-2 and MPP-2, up-regulate the expression of miR-34-5p, bax and E-cadherin; overexpression of miR-34-5p there were the same effect of the extract of the Actinidia root extract on the hepatocellular carcinoma cells, inhibition of miR-34-5p can reverse the proliferation, migration, invasion inhibition and apoptosis promotion of the Actinidia root extract hepatocellular carcinoma cells (F=34.118, 286.677, 253.530, 123.103, 103.396 and 109.770, P<0.05).

Actinidia root extract can inhibit the proliferation, migration, invasion and apoptosis of hepatocellular carcinoma cells, and its mechanism is related to the up-regulation of miR-34-5p.

Actinidia root extract; Hepatocellular carcinoma; MicroRNA-34-5p; Biological behavior