Experimental Study
Relationship between prognosis of Lumina B breast cancer and tumor infiltration of forxhead box protein 3+ regulatory T lymphocytes and CD8+ cytotoxic T lymphocytes
Peng Gongling, Wang Longqiang, Li Qiang, Jiang Xueqing
Published 2019-11-08
Cite as Chin J Exp Surg, 2019,36(11): 1975-1977. DOI: 10.3760/cma.j.issn.1001-9030.2019.11.016
Abstract
ObjectiveTo detect the expression level of forxhead box Protein 3 (FoxP3) and CD8 in invasive ductal carcinoma of breast Lumina B, investigate the relevance between FoxP3+ regulatory T lymphocyte (Treg) and CD8+ cytotoxic T lymphocyte (CTL) as well as prognosis of breast cancer patients.
MethodsImmunohistochemistry was used to detect the expression level of FoxP3 and CD8 in the paraffin-embedded breast cancer samples. One-sample t test was applied to analyze the relationship among the expression levels of FoxP3 and CD8 of patient’s age, tumor size, nodal status, and histophathologic grade. Kaplan meier Log-rank was used to analyze the correlation between the expression level of CD8 and FoxP3 and the overall survival and disease-free survival of breast cancer patients.
ResultsThe expression of CD8 is associated with tumor diameter ≤2 cm, lymph node negative metastasis, and with lower histopathologic grade (gradeⅠ/Ⅱ) (P<0.05). The level of FoxP3 is significantly related to tumor diameter >2 cm, lymph node metastasis (P<0.01). High expression level of FoxP3 infiltrating in tumor was could reduce the desease-free survival of breast cancer patients (P<0.05), while low expression of CD8 prolonged desease-free survival (P<0.01).
ConclusionTumor-infiltrating CD8+ CTLs are associated with good prognosis, whereas tumor-infiltrating FoxP3+ Tregs are associated with adverse outcomes.
Key words:
Invasive ductal carcinoma of breast; Tumor-infiltrating T lymphocyte; Regulatory T lymphocyte; Cytotoxic T lymphocytes; Forxhead box protein 3; CD8
Contributor Information
Peng Gongling
Department of Thyroid and Breast Surgery, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China
Wang Longqiang
Department of Thyroid and Breast Surgery, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China
Li Qiang
Department of Thyroid and Breast Surgery, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China
Jiang Xueqing
Department of Thyroid and Breast Surgery, the Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, China