Clinical Research
T-cell immunoglobulin and mucin domain 1 expression in human non-small-cell lung cancer tissues and its clinical significance
Li Min, Zheng Xiao, Fan Jialin, Xu Kai, Zhou You, Chen Lujun, Jiang Jingting
Published 2019-11-08
Cite as Chin J Exp Surg, 2019,36(11): 2093-2095. DOI: 10.3760/cma.j.issn.1001-9030.2019.11.051
Abstract
ObjectiveTo investigate the expression of T-cell immunoglobulin and mucin domain 1 (TIM-1) in non-small-cell lung cancer (NSCLC) tissues and to analyze its clinical significance. To further explore the regulatory effect of TIM-1 intervention on the biological function of NSCLC cell lines.
MethodsThe immunohistochemistry assay and the tissue micro-array were used to examine the TIM-1 expression in lung cancer tissues as well as adjacent normal lung tissues. The correlation between the expression level of TIM-1 and patients’ clinic pathological parameters was evaluated by using chi-square test. The lung cancer cell lines A549 and SK-MES-1 were used to construct the stable knockdown expression of TIM-1 using RNA interference (RNAi) method. The cell counting kit-8 (CCK-8), wound healing, and Transwell were used to examine the cellular function after TIM-1 knockdown of these cells.
ResultsHigher TIM-1 expression in lung squamous cell carcinoma tissues is significantly correlated with advanced TNM stage (Ⅲ+ Ⅳ: 33.3%, Ⅰ+ Ⅱ: 13.2%) (χ2=3.969, P<0.05), but not any other parameters. CCK-8 assay showed that, knockdown expression of TIM-1 make the cell proliferation rate decreased significantly. The wound healing assay showed that the cell migration abilities of LV-TIM-1-shRNA group was significantly lower than LV-NC group (A549, 24 h: t=17.540, P<0.01; SK-MES-1, 24 h: t=11.450, P<0.01). The Transwell assay showed that the cell invasion abilities was significantly decreased after knockdown expression of TIM-1.
ConclusionOur results showed that the TIM-1 was highly expressed in non-small-cell lung cancer tissues, and its expression level was significantly correlated with TNM stage in squamous cell carcinoma patients. TIM-1 expression in lung adenocarcinoma tissues could be used as independent prognostic predictor for the patients, while higher TIM-1 expression in lung squamous cell carcinoma tissues trends significant for the prognostic prediction for the patients. Knockdown expression of TIM-1 could inhibit the cell viability, and the abilities of migration and invasion, and thus TIM-1 could be used as potential therapeutic target for lung cancer.
Key words:
T-cell immunoglobulin and mucin domain 1; Lung cancer; Immunohistochemistry; RNA interference; Prognosis
Contributor Information
Li Min
The Third Affiliated Hospital of Soochow University, Jiangsu Engineering Research Center for Tumor Immunotherapy, Institute of Cell Therapy, Soochow University, Changzhou 213003, China
Zheng Xiao
The Third Affiliated Hospital of Soochow University, Jiangsu Engineering Research Center for Tumor Immunotherapy, Institute of Cell Therapy, Soochow University, Changzhou 213003, China
Fan Jialin
The Third Affiliated Hospital of Soochow University, Jiangsu Engineering Research Center for Tumor Immunotherapy, Institute of Cell Therapy, Soochow University, Changzhou 213003, China
Xu Kai
The Third Affiliated Hospital of Soochow University, Jiangsu Engineering Research Center for Tumor Immunotherapy, Institute of Cell Therapy, Soochow University, Changzhou 213003, China
Zhou You
The Third Affiliated Hospital of Soochow University, Jiangsu Engineering Research Center for Tumor Immunotherapy, Institute of Cell Therapy, Soochow University, Changzhou 213003, China
Chen Lujun
The Third Affiliated Hospital of Soochow University, Jiangsu Engineering Research Center for Tumor Immunotherapy, Institute of Cell Therapy, Soochow University, Changzhou 213003, China
Jiang Jingting
The Third Affiliated Hospital of Soochow University, Jiangsu Engineering Research Center for Tumor Immunotherapy, Institute of Cell Therapy, Soochow University, Changzhou 213003, China
