Orthopeadic Surgery
Injectable hydrogel combined with nucleus pulposus-derived mesenchymal stem cell for the treatment of degenerative intervertebral disc in rats
Wang Feng, Zhang Wenjie, Feng Xinmin, Nan Liping, Zhou Shifeng, Liu Yang, Wang Shuguang, Chen Dong, Cai Tongchuan, Zhang Liang
Published 2019-11-08
Cite as Chin J Exp Surg, 2019,36(11): 1949-1952. DOI: 10.3760/cma.j.issn.1001-9030.2019.11.008
Abstract
ObjectiveTo investigate the effect of injectable hydrogel combined with nucleus pulposus-derived mesenchymal stem cell (NPMSC) transplantation on the degenerative intervertebral disc in rats.
MethodsNPMSC was isolated from the caudal spinal nucleus pulposus of Sprague-Dawley rats and identification of mesenchymal stem cells was determined. After 2 weeks of intervertebral disc degeneration model was established by annulus fibrosus puncture, the groups were divided: control group (untreated), treatment group (injection of hydrogel loaded NPMSC into the intervertebral disc), phosphate buffer solution (PBS) group (injection of the same amount of PBS), hydrogel group (injection of the same amount of hydrogel), cell group (injection of the same amount of NPMSC suspension). At 4 and 8 weeks after intervention, relative disc height index (DHI%) and magnetic resonance imaging (MRI) index were detected. At 8 weeks after intervention, histological score, the expression of collagen type Ⅱ and aggrecan was detected by immunofluorescence and reverse transcription-polymerase chain reaction. One-way ANOVA was used for comparison between groups, and LSD-t test was used for pairwise comparison.
ResultsAt 8 weeks after intervention, for the DHI% and MRI index, the experiment group [(87.80±3.40)%, 3 313.84±227.80] was higher than cell group [(79.65±2.33)%, 3 006.83±147.92] (t=3.411, 3.127, P<0.05); cell group was higher than hydrogel group [(69.49±4.79)%, 1 288.00±202.09] (t=4.252, 17.504, P<0.05); for histological score, the cell group (9.00±1.29) was higher than experiment group (7.16±0.68) (t=3.550, P<0.05), the hydrogel group (12.50±0.95) was higher than cell group (t=6, 753, P<0.05); for the protein express of collagen type Ⅱ and aggrecan, the cell group (0.30±0.02, 0.58±0.03) was less than experiment group (0.38±0.03, 0.69±0.05) (t=-4.000, -3, 267, P<0.05), the hydrogel group (0.14±0.03, 0.14±0.04) was less than cell group (t=-8.000, -13.307, P<0.05).
ConclusionInjectable hydrogel combined with NPMSC transplantation can delay the level of disc degeneration in animal model and promote the repair and regeneration of degenerative disc.
Key words:
Hydrogel; Nucleus pulposus-derived mesenchymal stem cell; Intervertebral disc degeneration; Cell transplantation
Contributor Information
Wang Feng
Graduate School, Dalian Medical University, Dalian 116000, China; Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, China
Zhang Wenjie
Department of Orthopedics, International Zhuang Hospital, Guangxi University of Traditional Chinese Medicine, Nanning 530001, China
Feng Xinmin
Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, China
Nan Liping
Graduate School, Dalian Medical University, Dalian 116000, China; Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, China
Zhou Shifeng
Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, China
Liu Yang
Graduate School, Dalian Medical University, Dalian 116000, China; Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, China
Wang Shuguang
Graduate School, Dalian Medical University, Dalian 116000, China; Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, China
Chen Dong
Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, China
Cai Tongchuan
Graduate School, Dalian Medical University, Dalian 116000, China; Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, China
Zhang Liang
Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, China
