Experimental Study
Fibroblast activation induced by common bile duct ligation in rats with obstructive jaundice
Zhang Qinghua, Ou Guixue, Wang Ruihua, Yu Zhenxing, Liu Jingfeng
Published 2020-01-08
Cite as Chin J Exp Surg, 2020,37(01): 60-62. DOI: 10.3760/cma.j.issn.1001-9030.2020.01.017
Abstract
ObjectiveTo investigate the effect of common bile duct ligation on fibroblast activation in rats with obstructive jaundice.
MethodsTwenty-four male specific pathogen free (SPF) rats were randomly divided into sham-operated group and experimental group, with 12 rats in each group. All rats underwent common bile duct ligation. Rats The common bile duct in the experimental group was ligated with 3-0 silk thread and cut off. The rats in the two groups were sampled 2 and 7 days after operation. Enzyme-linked immunosorbent assay (ELISA) was used to detect the content of transforming growth factor-β1 (TGF-β1). Immunohistochemistry was used to detect the expression of TGF-β1 protein. The real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect the expression of TGF-β1 gene. Western blotting was used to detect the expression of TGF-β1 protein.
ResultsIn sham-operated group, the structure of hepatic lobules was intact, and the hepatic sinuses were normal without congestion. In experimental group, the structure of hepatic lobules was disordered and the hepatic cords were dissociated. The level of serum TGF-β1 in the experimental group at 2nd day [(689.42±58.13) ng/L] and 7th day [(867.53±45.62) ng/L] was significantly higher than that in the sham-operated group [(519.92±32.41) ng/L and (536.50±41.29) ng/L, t=8.822 and 18.635, P<0.05]. The expression of TGF-β1 protein in liver tissues of experimental group at 2nd day [(6.53±1.29)%] and 7th day [(8.97±1.85)%] was significantly higher than that of sham-operated group [(1.17±0.24)% and (1.54±0.39)%, t=8.822 and 18.635, P<0.05]. The expression of TGF-β1 in liver tissues of experimental group at 2nd day [(0.89±0.14)%] and 7th day [(0.95±0.19)%] was significantly higher than that of sham-operated group [(0.52±1.32)% and (0.31±1.76)%, t=14.693 and 18.316, P<0.05]. The gray value of TGF-β1 protein expression in liver tissue of experimental group at 2nd day after operation (0.62±0.13) and 7th day after operation (0.78±0.10) was significantly higher than that of sham-operated group (0.21±0.05 and 0.23±0.06, t=10.197 and 16.337, P<0.05).
ConclusionThe expression of TGF-β1 in rats with obstructive jaundice induced by common bile duct ligation is significantly up-regulated, and the proliferation of biliary duct epithelial cells induces fibroblast activation.
Key words:
Common bile duct ligation; Obstructive jaundice; Fibroblast activation
Contributor Information
Zhang Qinghua
Department of General Surgery, Mindong Hospital Affiliated to Fujian Medical University, Fu’an 355000, China
Ou Guixue
Department of General Surgery, Mindong Hospital Affiliated to Fujian Medical University, Fu’an 355000, China
Wang Ruihua
Department of General Surgery, Mindong Hospital Affiliated to Fujian Medical University, Fu’an 355000, China
Yu Zhenxing
Department of General Surgery, Mindong Hospital Affiliated to Fujian Medical University, Fu’an 355000, China
Liu Jingfeng
Department of Hepatobiliary Surgery, Mengchao Hepatobiliary Hospital of Fujian Medical University, Fuzhou 350025, China
