Monographic Research • Trauma Repair and Scar Plastic
Expression and clinical significance of autophagy-related proteins LC3, P62 and Beclin-1 in hypertrophic scar formation
Xiaoxia Chen, Siwei Qu, Xiao Han, Zizi Chen, Jia Chen, Ping Li, Chaoqi Yin, Sainan Zeng, Aijun Wang, Bin He, Honghui Su, Jianda Zhou
Published 2019-04-20
Cite as J Chin Physician, 2019, 21(4): 489-494. DOI: 10.3760/cma.j.issn.1008-1372.2019.04.003
Abstract
ObjectiveTo explore the differences in autophagic expression levels between hypertrophic scar (HS) tissue and normal skin tissue, and further investigate the relationship between hypertrophic scar formation and autophagy protein expression through the rabbit ear hypertrophic scar model.
Methods30 patients with hypertrophic scar were collected. One hypertrophic scar tissue and one normal skin tissue were harvested. The relative expressions of LC3, P62 and Beclin-1 in each tissue specimen were detected by immunohistochemistry and Western blot. Western blot was used to detect the autophagic-associated protein LC3 (MAPLC3), P62 and Beclin-1 in the hypertrophic scar tissue of rabbit ear and the corresponding normal tissue of rabbit ears at 4 weeks, 8 weeks, 12 weeks, and 24 weeks, and further explore their clinical significance.
ResultsIn vivo, the expression of hypertrophic scar tissue protein LC3 and Beclin-1 was significantly stronger than that in normal skin tissue (P<0.05). The expression of P62 was significantly weaker than that in normal skin tissue (P<0.05). In animal experiments, during the process of HS formation, the protein expression of LC3 gradually increased , while the protein expression of P62 gradually decreased; the protein expression of Beclin-1 was higher than that of normal rabbit ears tissue, with statistically significant differences (P<0.05).
ConclusionsThe expression of LC3 and Beclin-1 in human hypertrophic scar tissues is higher than that in normal tissues. While the expression of P62 is lower than that in normal tissues. That is, the expression of autophagy in human hypertrophic scar tissue showed an upward trend in a certain period of time, and was significantly higher than that in normal tissue.
Key words:
Cicatrix, hypertrophic; Microtubule-associated proteins; Beclin-1; RNA-binding proteins; Autophagy; Rabbits
Contributor Information
Xiaoxia Chen
Department of Plastic and Cosmetic Surgery, the People′s Hospital of Ningxiang, Ningxiang 410600, China
Department of Plastic and Cosmetic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, China
Siwei Qu
Department of Plastic and Cosmetic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, China
Xiao Han
Department of Plastic and Cosmetic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, China
Zizi Chen
Department of Plastic and Cosmetic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, China
Jia Chen
Department of Plastic and Cosmetic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, China
Ping Li
Department of Plastic and Cosmetic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, China
Chaoqi Yin
Department of Plastic and Cosmetic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, China
Sainan Zeng
Department of Plastic and Cosmetic Surgery, the People′s Hospital of Ningxiang, Ningxiang 410600, China
Aijun Wang
Surgical Bioengineering Laboratory, Department of Surgery, UC Davis School of Medicine, University of California, Sacramento, California 95817, USA
Bin He
Department of Plastic and Cosmetic Surgery, the People′s Hospital of Ningxiang, Ningxiang 410600, China
Department of Plastic and Cosmetic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, China
Honghui Su
Department of Plastic and Cosmetic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, China
Jianda Zhou
Department of Plastic and Cosmetic Surgery, the Third Xiangya Hospital of Central South University, Changsha 410013, China
