Original Article
Effect of minimally invasive drilling closed drainage on the expression of HIF-1α, Claudin-5, TGF- β and Smad2/3 in patients with chronic subdural hematoma
Gao Xuejun, Zeng Jingbo, Zhao Qiang, Ma Dongbin
Published 2019-09-20
Cite as J Chin Physician, 2019,21(9): 1345-1348. DOI: 10.3760/cma.j.issn.1008-1372.2019.09.014
Abstract
ObjectiveTo investigate the effects of single-hole and double-hole drilling and closed drainage on the expression of Hypoxia inducible factor-1α (HIF-1α), Claudin-5, transforming growth factor-β (TGF-β) and Smad2/3 in the epidermis of patients with chronic subdural hematoma.
Methods100 patients with chronic subdural hematoma were randomly divided into single hole and double hole drainage group according to random number table. Immunohistochemical streptavidin-peroxidase (SP) was used to detect the expression of TGF- β protein, and Western blot was used to detect the expression of HIF-1α, Claudin-5 and Smad2/3 in the epihematoma of the two groups before and after operation.
ResultsCompared with pre-operation, the expression of HIF-1α, TGF-β and Smad2/3 protein in adventitia of hematoma in both groups decreased after operation, especially in the double-hole group (P<0.05). The expression of Claudin-5 protein in adventitia of hematoma was significantly increased in both groups, especially in the double-hole group, with statistically significant difference (P<0.05).
ConclusionsSingle-hole and double-hole drilling and sealing drainage is an effective method to treat chronic subdural hematoma. It can reduce the expression of HIF-1α, TGF-β and Smad2/3 protein in the epidermis of hematoma, and significantly increase the expression of Claudin-5, and the effect of double-hole drilling and sealing drainage is more significant.
Key words:
Drainage; Hematoma, subdural, chronic; Hypoxia-inducible factor 1, alpha subunit; Claudin-5; Transforming growth factor beta; Smad proteins, receptor-regulated
Contributor Information
Gao Xuejun
Department of Neurosurgery, the fifth People′s Hospital of Chengdu, Chengdu 611130, China
Zeng Jingbo
Zhao Qiang
Ma Dongbin