Basic Study
Peritoneal dialysis improves lung function in rats with lung injury induced by shock wave
Wei Hu, Kehong Chen, Xiaoyue Wang, Juan Du, Yani He
Published 2019-09-15
Cite as Chin J Nephrol, 2019, 35(9): 684-689. DOI: 10.3760/cma.j.issn.1001-7097.2019.09.007
Abstract
ObjectiveTo prove the efficacy of peritoneal dialysis on shock wave-induced acute lung injury of rats, and analyze its mechanisms.
MethodsForty-five adult Sprague-Dawley rats were randomly divided into three groups: control group, sham operation (Sham) group and peritoneal dialysis (PD) group. Sham group and PD group did abdominal catheterization before blast injury. The 55 kg shock wave (bst-I) was used to induce lung blast injury. After one hour of blast injury, PD group was given 2.5% peritoneal dialysate 20 ml to stay abdomen, which was released 30 min posted, repeated 12 cycles. After 6 hours of peritoneal dialysis, all of the rats were sacrificed. Partial damaged tissues in lung were used to evaluate the pathomorphologic changes by HE staining, and the remnants were used to measure the lung water content. Lung function was detected by blood gas analyzer and small animal detector from the arterial blood gas. The levels of serum inflammatory factors, such as tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6 and monocyte chemoattractant protein-1 (MCP-1) were tested by ELISA.
ResultsThe relative integrity of alveolar structure, interstitial edema and inflammatory cell infiltration in PD group were significantly improved than those in control group. The lung water content of PD group was significantly lower than that of control group (P<0.05). The levels of TNF-α, IL-1β, IL-6 and MCP-1 in serum of PD group were significantly lower than those in control group (all P<0.05). The blood oxygen saturation, oxygen partial pressure, oxygenation index, vital capacity, functional residual volume and maximum mid-expiratory flow rate in PD group were significantly higher than those in control group (all P<0.05).
ConclusionsThrough reducing pulmonary edema and inflammatory factors, peritoneal dialysis can improve lung function in shock wave -induced acute lung injury of rats.
Key words:
Peritoneal dialysis; Lung injury; Cytokines; Inflammation; Respiratory function tests
Contributor Information
Wei Hu
Department of Nephrology, Army Characteristic Medical Center, Chongqing 400010, China
Department of Nephrology, Army 958 Hospital, Chongqing 400020, China
Kehong Chen
Department of Nephrology, Army Characteristic Medical Center, Chongqing 400010, China
Xiaoyue Wang
Department of Nephrology, Army Characteristic Medical Center, Chongqing 400010, China
Juan Du
Department of NO.4 Field Surgery, Army Special Medicine Center, Chongqing 400010, China
Yani He
Department of Nephrology, Army Characteristic Medical Center, Chongqing 400010, China