Scientific Research
Application of microcolumn gel cartridge method in the detection of immunological antibody potency in pregnant women
Lyu Xiaoying, Chen Yongquan, Wang Houzhao, Li Lingbo
Published 2017-06-15
Cite as IMHGN, 2017,27(12): 1832-1834. DOI: 10.3760/cma.j.issn.1007-1245.2017.12.005
Abstract
ObjectiveTo explore the effect of microcolumn gel cassette method in prenatal detection of immunological antibody potency in pregnant women.
MethodsThe anti-A and anti-B potency of 180 cases of O-type pregnant women (95 cases of their partners were A type and the other 85 cases were B type) were detected by both the card method and the test tube anti-human globulin method, and the difference in sensitivity between them were compared.
ResultsThe mean value of anti-A potency by the card method and the test tube anti-human globulin method were 46.21 and 33.82 respectively, the former was 1.37 times higher than the latter. The mean value of anti-B potency by the card method and the test tube anti-human globulin method were 47.18 and 35.02 respectively, the former was 1.35 times higher than the latter. To make IgG anti-A (anti-B) potency ≥64 to be positive, the positive cases of IgG anti-A by the card method and the test tube anti-human globulin method were 45 (47.37%) and 8 (8.42%) respectively, the former was higher than the latter, with statistically significant difference (degree of freedom = 1, χ2 = 33.23, P<0.01). The positive cases of IgG anti-B by the card method and the test tube anti-human globulin method were 38 (44.71%) and 10 (11.76%) respectively, the former was higher than the latter, with statistically significant difference (degrees of freedom = 1, χ2 = 24.30, P<0.01).
ConclusionThe sensitivity of card method in prenatal detection of the antibody potency of pregnant women is higher than that of test tube anti-human globulin method, with the simple method, and easy to be standardized, worthy to be popularized.
Key words:
Card method; Test tube anti-human globulin method; Immunological antibody; Potency
Contributor Information
Lyu Xiaoying
Clinical Laboratory, The 174th Hospital of the Chinese People's Liberation Army, Xiamen 361003, China
Chen Yongquan
Clinical Laboratory, The 174th Hospital of the Chinese People's Liberation Army, Xiamen 361003, China
Wang Houzhao
Clinical Laboratory, The 174th Hospital of the Chinese People's Liberation Army, Xiamen 361003, China
Li Lingbo
Changchun Bo Xun Biotechnology Limited Liability Company, Changchun 130102, China
