Scientific Research
Effect of Dezocine on the action potential of sciatic nerve stem of rats
Xie Changchun, Chen Xu, Liu Cuicui, Xin Wenjun
Published 2017-08-15
Cite as IMHGN, 2017,23(16): 2524-2528. DOI: 10.3760/cma.j.issn.1007-1245.2017.16.007
Abstract
ObjectiveThe electrophysiological techniques were applied to investigate the effect of dezocine on the threshold intensity, the optimal intensity, and the conduction velocity of compound action potential of sciatic nerve stem of rats. It may provide a potential theoretical basis for the mechanism of dezocine action on the peripheral nerve system.
MethodsAfter incubating with normal saline for 10 min, the sciatic nerve stems of rats were divided into five groups (0.25 mg/ml, 2.5 mg/ml, and 5.0 mg/ml dezocine, 0.25% lidocaine group, 5.0 mg/ml dezocine + 0.25% lidocaine group). Before and after the treatment for 20 min, the threshold intensity, the optimal intensity, and the conduction velocity of compound action potential of sciatic nerve stems were examined by BL-420 + biological function experimental signal analysis system. There after, 5.0 mg/ml dezocine-treated sciatic nerve stems were then incubated in three different concentration of naloxone (0.02 mg/ml, 0.2 mg/ml, or 0.4 mg/ml) for 20 min, thus to observe the alteration of threshold intensity, optimal intensity, and conduction velocity of compound action potential.
ResultsCompared with the control group, dezocine treatment at dosage of 2.5 mg/ml and 5.0 mg/ml, but not 0.25 mg/ml obviously induced upregulation of threshold intensity and optimal intensity of compound action potential, and downregulation of the amplitude and conduction velocity of compound action potential of sciatic nerve stems (P<0.05). Naloxone treatment at dosage of 0.2 mg/ml and 0.4 mg/ml, but not 0.02 mg/ml significantly attenuated the increase of threshold intensity and optimal intensity of compound action potential, and the decrease of amplitude and conduction velocity of compound action potential induced by dezocine application. Compared with 0.25% lidocaine treatment group, 5.0 mg/ml dezocine and 0.25% lidocaine treatment showed cooperative effect on inhibiting the action potential of sciatic nerve stem.
ConclusionsDezocine treatment significantly reduced the excitability and the conduction velocity of compound action potential of sciatic nerve stem in vitro, which can be rescued by naloxone application. Moreover, dezocine treatment also obviously accelerates the effectiveness of lidocaine.
Key words:
Dezocine; Sciatic nerve; Action potential; Threshold intensity; Optimal intensity; Conduction velocity
Contributor Information
Xie Changchun
Department of Anesthesiology, Liwan Hospital of Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510170, China
Chen Xu
Department of Anesthesiology, Liwan Hospital of Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510170, China
Liu Cuicui
Department of Physiology, Zhongshan Medical School of Sun Yat-Sen University, Guangzhou 510060, China
Xin Wenjun
Department of Physiology, Zhongshan Medical School of Sun Yat-Sen University, Guangzhou 510060, China
