Scientific Research
The effect of Chinese medicine hair-restoring lotion on proliferation in vitro of human epiderm keratinocytes
Lin Zhuangmin, Kong Xiujuan, Shen Xiaohua, Wu Qingguang, Zhang Lingling, Zhang Rongwen, Li Geng, Wu Yina
Published 2018-08-01
Cite as IMHGN, 2018,24(15): 2237-2240. DOI: 10.3760/cma.j.issn.1007-1245.2018.15.005
Abstract
ObjectiveTo study the effect of Chinese medicine hair-restoring lotion on proliferation in vitro of human epiderm keratinocytes (HEK).
MethodsTaking Zhangguang 101 hair-restoring lotion as a positive drug, cultured the HEK by using enzymatic digestion, observed the morphology of the cells, determined the growth activity of HEK by MTT assay, and drew the growth curve of cells of each group, to evaluate the Chinese medicine hair-restoring lotion on HEK proliferation in vitro.
ResultsCompared with the blank control group, administrated 24 h later, the cells of Chinese medicine hair-restoring lotion group increased in number, some cells were connected to each other, there was no transparent vacuoles in cytoplasm, the size of cells was uniform, and the growth condition was good; MTT results showed that the OD value of each dose Chinese medicine hair-restoring lotion group increased significantly (P<0.01), and the promotion rate of the proliferation of HEK was above 40%.
ConclusionEach dose group of Chinese medicine hair-restoring lotion can significantly promote the proliferation of HEK in vitro.
Key words:
Chinese medicine hair-restoring lotion; Human epiderm keratinocytes (HEK); MTT
Contributor Information
Lin Zhuangmin
Department of Pharmacy, Guangdong Second Provincial General Hospital, Guangzhou 510317, China
Kong Xiujuan
Guangzhou University of Chinese Medicine, Guangzhou 510006, China
Shen Xiaohua
Guangzhou University of Chinese Medicine, Guangzhou 510006, China
Wu Qingguang
Guangzhou University of Chinese Medicine, Guangzhou 510006, China
Zhang Lingling
Guangzhou University of Chinese Medicine, Guangzhou 510006, China
Zhang Rongwen
King (Guangzhou) Co., Ltd., Guangzhou 510000, China
Li Geng
Guangzhou University of Chinese Medicine, Guangzhou 510006, China
Wu Yina
Guangzhou University of Chinese Medicine, Guangzhou 510006, China
