Other Liver Disease
Study of reactive oxygen species and adiponectin for chronic HBV infection combined with nonalcoholic fatty liver diseases
Xu Liang, Zhong Yan, Su Shuting, Liu Yonggang, Lyu Feinan, Zhou Xiaoli, Ren Jinqing, Li Ping, Shi Ruifang, Jiang Yong, Fan Jiangao, Mi Yuqiang
Published 2020-03-20
Cite as Chin J Hepatol, 2020,28(03): 247-253. DOI: 10.3760/cma.j.cn501113-20191231-00486
Abstract
ObjectiveTo investigate the application value of reactive oxygen species (ROS) and adiponectin (ADPN) in the judgment of liver inflammation in chronic hepatitis B virus infection combined with nonalcoholic fatty liver disease (NAFLD).
MethodsA total of 159 cases with NAFLD (21 cases), chronic hepatitis B virus infection (57 cases), and chronic hepatitis B virus infection combined with NAFLD (81 cases) were collected between June 2016 to December 2018, and the visited patients diagnosis were confirmed by histopathological examination of the liver. ROS and ADPN level retained in serum was determined by enzyme-linked immunosorbent assay. Histopathological examination of liver tissue was used as the gold standard to discuss the diagnostic value of the serum in patients with chronic hepatitis B virus infection combined with NAFLD for the occurrence of nonalcoholic steatohepatitis. One-way analysis of variance was used for the comparison among multiple groups, and LSD-t test was used for pairwise comparison between groups. Measurement data for non-normal distributions were expressed as M (P25, P75). Comparisons between groups were performed using the Mann-Whitney U or Kruskal-Wallis H test. Chi-square test was used to compare the count data between groups. Correlation analysis was performed using Spearman correlation analysis. Histopathological grouping of liver tissue was used as the gold standard, and the area under the receiver operating characteristic curve was used to evaluate the diagnostic efficacy of the regression formula.
Results(1) In patients with chronic hepatitis B virus infection combined with NAFLD, the levels of ROS in the non-hepatic steatosis group and the mild hepatic steatosis group were significantly lower than those in the moderate and severe hepatic steatosis group, while the ADPN level in the non-hepatic steatosis group was significantly higher than liver steatosis group, P < 0.05. (2) The results of correlation analysis showed that ROS was significantly correlated with NAS score, change in the degree of fatty liver and lobular inflammation (all P < 0.05).There was a significant negative correlation between ADPN and the change in the degree of fatty liver (P < 0.05). (3) Logistic regression analysis results showed that the diagnostic formula for chronic hepatitis B virus infection combined with nonalcoholic steatohepatitis was 0.02 × controlled attenuation index + 0.584 × white blood cells/109 + 0.587 × ROS-10.982. The area under receiver operating characteristic curve of the subject was = 0.896. The sensitivity, specificity, positive and negative predictive value were 97.1%, 71.2%, 64.2%, and 97.9%.
ConclusionADPN and ROS have certain reference value in differentiating the change in the degree of fatty liver and inflammation in chronic hepatitis B virus infection combined with NAFLD and the diagnostic formula has higher application value in the diagnosis and exclusion of chronic hepatitis B virus infection combined with nonalcoholic steatohepatitis.
Key words:
Oxides; Adiponectin; Hepatitis B virus; Fatty liver, non-alcoholic; Inflammation
Contributor Information
Xu Liang
Tianjin Second People's Hospital, Tianjin Institute of Hepatology, Tianjin 300192, China
Zhong Yan
Yanqing County Hospital of Beijing (Yanqing hospital, the Third Hospital of Peking University), Beijing 102100, China
Su Shuting
Tianjin Second People's Hospital, Tianjin Institute of Hepatology, Tianjin 300192, China
Liu Yonggang
Tianjin Second People's Hospital, Tianjin Institute of Hepatology, Tianjin 300192, China
Lyu Feinan
Tianjin Second People's Hospital, Tianjin Institute of Hepatology, Tianjin 300192, China
Zhou Xiaoli
Tianjin Second People's Hospital, Tianjin Institute of Hepatology, Tianjin 300192, China
Ren Jinqing
Tianjin Second People's Hospital, Tianjin Institute of Hepatology, Tianjin 300192, China
Li Ping
Tianjin Second People's Hospital, Tianjin Institute of Hepatology, Tianjin 300192, China
Shi Ruifang
Tianjin Second People's Hospital, Tianjin Institute of Hepatology, Tianjin 300192, China
Jiang Yong
Department of Gastroenterology, The Second Hospital of Tianjin Medical University, Tianjin 300211, China
Fan Jiangao
Center for Fatty Liver, Department of Gastroenterology, Xinhua Hospital, Shanghai Jiaotong University STCol of Medicine, Shanghai 200092, China
Mi Yuqiang
Tianjin Second People's Hospital, Tianjin Institute of Hepatology, Tianjin 300192, China