Study of gene correlation between endothelial growth factor family in mouse eye
Cui Jinglin, Lu Hang, Chen Jing, Luo Binjiang, Xiu Weiwei, Li Huimei, Huang Jiao, Chen Yuhan, Chen Jingyu
Published 2020-03-25
Cite as Chin J Ocul Fundus Dis, 2020,36(03): 211-219. DOI: 10.3760/cma.j.cn511434-20180803-00269
Abstract
ObjectiveTo compare the function and action pathways of VEGFA, VEGFB and VEGFC in VEGF family of mouse eye.
MethodsUsing the BXD mouse gene data in Genenetwork database as template to compare and study the similarities and differences of VEGFA, VEGFB and VEGFC molecular pathways or potential functions in the whole genome expression spectrum of BXD recombinant mouse inbred line population, with multiple analytical methods and statistical strategies were used, such as gene expression level, target genes comparison, top genes comparison associated to target genes, expression Quantitative Trait Loci (eQTL).
ResultsMatrix comparison showed strong positive correlation between two probes of VEGFC (r=0.732, P<0.01), weak correlation between VEGFA 1420909 and VEGFC 1440739, VEGFA 1451959 and VEGFC 1451803, VEGFC 1419417959 and VEGFC 1439766, VEGFC 1451803 and VEGFC 1439766 (P<0.05); there was no correlation between VEGFA 1420909 and four other genes except VEGFC 1440739, VEGFA 1451959 and VEGFC 1440739, VEGFB 1451803 and VEGFA 1420909/VEGFC 1419417/VEGFC 1440739 (P >0.05). In the comparative analysis of the relevant Top50 genes of each VEGF gene, most of the genes in BXD mouse were not significantly correlated with VEGFA, VEGFB and VEGFC except for the weak association of individual related genes. The results of eQTL analysis showed that each probe of VEGF gene was located on different chromosomes.
ConclusionsThe expression levels and positive and negative correlations of VEGFA, VEGFB and VEGFC were different in the VEGF family of mouse eye, suggesting that these genes may play their role through different pathways.
Key words:
Vascular endothelial growth factors; DNA probes; Genome components
Contributor Information
Cui Jinglin
Department of Ophthalmology, The First Hospital of Qiqihar in Heilongjiang Province, Qiqihar 161005, China
Lu Hang
Department of Ophthalmology, The First Hospital of Qiqihar in Heilongjiang Province, Qiqihar 161005, China
Chen Jing
Department of Ophthalmology, The First Hospital of Qiqihar in Heilongjiang Province, Qiqihar 161005, China
Luo Binjiang
Department of Ophthalmology, The First Hospital of Qiqihar in Heilongjiang Province, Qiqihar 161005, China
Xiu Weiwei
Department of Ophthalmology, The First Hospital of Qiqihar in Heilongjiang Province, Qiqihar 161005, China
Li Huimei
Department of Ophthalmology, The First Hospital of Qiqihar in Heilongjiang Province, Qiqihar 161005, China
Huang Jiao
Department of Ophthalmology, The First Hospital of Qiqihar in Heilongjiang Province, Qiqihar 161005, China
Chen Yuhan
Guanganmen Hospital, Chinese Academy of Traditional Chinese Medicine, Beijing 100053, China
Chen Jingyu
The First Clinical Medical College of Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China
