The protective effect of Zhicao Tea Mixture on Müller cells and the expression of inflammatory factors in mice with diabetic retinopathy
Zhu Manhui, Tu Yuanyuan, Wang Zhenzhen, Du Jingxia, Guo Yang, Xu Jiaowen, Song E
Published 2020-04-25
Cite as Chin J Ocul Fundus Dis, 2020,36(04): 302-307. DOI: 10.3760/cma.j.cn511434-20191203-00397
Abstract
ObjectiveTo observe the protective effect of Zhicao Tea Mixture on Müller cells and the expression of inflammatory factors in mice with diabetic retinopathy.
MethodsSeventy-five C57BL/6J mice were randomly divided into the normal control group, diabetes mellitus (DM) group, low concentrations group, medium concentrations group and high concentrations group, with 16 mice in each group. The diabetes model of mice in all groups except the normal control group were established by intraperitoneal injection of STZ (60 mg/kg). Four weeks after the successful modeling, the Zhicao Tea Mixture with low (30 ml/kg), medium (60 ml/kg) and high concentrations (120 ml/kg) were respectively administered by gavage. Weight and blood glucose of mice in each group were measured every two weeks. After 8 weeks, Western blot method was used to detect the mice retina Müller cells activation marker gelatinous fibrous acidic protein (GFAP). Immunofluorescence was performed to detect the expression GFAP and glutamine synthetase (GS). Real-time quantitative PCR (RT- qPCR) and ELISA were used to determine the mRNA and protein expression levels of mouse retinal VEGF, TNF-α, IL-1β and IL-6 respectively.
ResultsThe weight of mice in the DM group was lower than that of the normal control group, and the blood glucose was increased. Zhicao Tea Mixture had no effect on the weight of DM mice, but had a significant hypoglycemic effect. The GFAP expression (t=38.318, P<0.001) in the retina of mice in the DM group was increased and GS expression (t=29.737, P<0.001) was decreased compared with the control group. The GFAP expression (t=13.677, 19.387, 16.305; P<0.05) in the retina of mice in the low, medium and high concentrations group were decreased and GS expression (t=5.170, 19.399, 6.705; P<0.05) were increased compared with the DM group. The expressions of retinal inflammatory factors VEGF, TNF-α, IL-1β and IL-6 in DM group all increased, while the expressions of the above-mentioned inflammatory factors in the retina of mice decreased in the low, medium and high concentrations group.
ConclusionZhicao Tea Mixture can decrease the blood glucose of DM mice and reduces the diabetic retinal inflammatory response.
Key words:
Diabetic retinopathy/physiopathology; Inflammation; Müller cells; Zhicao Tea Mixture
Contributor Information
Zhu Manhui
Lixiang Eye Hospital of Soochow University, Suzhou 215021, China
Tu Yuanyuan
Lixiang Eye Hospital of Soochow University, Suzhou 215021, China
Wang Zhenzhen
Lixiang Eye Hospital of Soochow University, Suzhou 215021, China
Du Jingxia
Lixiang Eye Hospital of Soochow University, Suzhou 215021, China
Guo Yang
Lixiang Eye Hospital of Soochow University, Suzhou 215021, China
Xu Jiaowen
Lixiang Eye Hospital of Soochow University, Suzhou 215021, China
Song E
Lixiang Eye Hospital of Soochow University, Suzhou 215021, China
