Technical Method
Comparison of nucleic acid detections between throat swab and stool specimens in 2019-nCoV infected patients
Li Bo, Zhang Hao, Li Boan, Chen Zhu
Published 2022-04-28
Cite as Chinese J Exp Clin Virol, 2022, 36(2): 236-238. DOI: 10.3760/cma.j.cn112866-20200303-00041
Abstract
ObjectiveTo evaluate the positive rate of nucleic acid detections in throat swab and stool specimens of patients, thereby to provide a reference to the clinical laboratory diagnosis.
MethodsForty-one confirmed patients were enrolled in this study, their throat swab and stool specimens were collected for detections. All the samples were determined by a fluorescence quantitative PCR method . The positive rate and concordance were evaluated for these two types of samples.
ResultsAmong those 41 patients, 9 of them had positive result in throat swab samples, accounted for 30%. Five patients had positive result in stool samples, accounted for 12.2%. Only two patients had concordant positive result . Kappa tests showed that the concordance was poor, kappa index was 0.153.
ConclusionsThe nucleic acid detection in stool specimen is a complemental method for respiratory tract samples and therefore may provide. additional information for infectiousness of patients.
Key words:
2019-nCoV; Reverse transcriptase polymerase chain reaction; Throat swab; Stool
Contributor Information
Li Bo
The Clinical Laboratory, Department of the Fifth Medical Centre of the People’s Liberation Army General Hospital, Beijing 100039, China
Zhang Hao
The Clinical Laboratory, Department of the Fifth Medical Centre of the People’s Liberation Army General Hospital, Beijing 100039, China
Li Boan
The Clinical Laboratory, Department of the Fifth Medical Centre of the People’s Liberation Army General Hospital, Beijing 100039, China
Chen Zhu
The Clinical Laboratory, Department of the Fifth Medical Centre of the People’s Liberation Army General Hospital, Beijing 100039, China
