Original Article
Relationship between Neurolingin - 4 Gene Single Nucleotide Polymorphisms and Childhood Autism
MA Dan, WANG Li-ping, GUO Lan-min, LV Zhi-hai, TIAN Shi-jie, JIANG Zhi-mei
Published 2010-04-20
Cite as J Appl Clin Pediatr, 2010, 25(8): 584-586. DOI: 10.3760/cma.j.issn.2095-428X.2010.08.119
Abstract
ObjectiveTo investigate the relationship between single nucleotide polymorphisms (SNPs) of neurolingin - 4 (NLGN4X) gene and childhood autism in Chinese han population.
MethodsPolymerase chain reaction - restricted fragment length polymorphism (PCR - RFLP) was used to determine allele and genotype of 2 SNPs (rs7049300 and rs1882260) of NLGN4X in 40 Chinese Han autism children (autism group) and 40 healthy children (healthy control group). Using statistical software SPSS 12.0, the distribution of SNPs Loci allele were analyzed through case - control study, and SNPs loci of autism group and the healthy control group were tested regarding Hardy - Weinberg equilibrium tests.
ResultsThe genotype distributions of the SNPs (rs7049300 and rs1882260) in autism group and healthy control group were consistent with the Hardy - Weinberg equilibrium (χ2=0.008, 0.144, Pa>0.05). There was not significant difference in the distribution of the allelic frequency and genotype between the two groups [rs7049300: χ2 (genotype frequency)=1.382, χ2 (allelic frequency)=0.928, Pa> 0.05; rs1882260: χ2 (genotype frequency)=0.811, χ2 (allelic frequency)=0.921, Pa>0.05].
ConclusionsThe polymorphisms of rs7049300 and rs1882260 in the NLGN4X are not associated with childhood autism, etiological genes of children autism need further research.
Key words:
autism; neurolingin - 4 gene; polymorphism; restricted fragment length
Contributor Information
MA Dan
Department of Child Development and Behavior, the Third Affiliated Hospital of Jiamusi University/Children Neurological Rehabilitation Lab, Jiamusi University, Jiamusi 154003, Heilongjiang Province, China
WANG Li-ping
Department of Child Development and Behavior, the Third Affiliated Hospital of Jiamusi University/Children Neurological Rehabilitation Lab, Jiamusi University, Jiamusi 154003, Heilongjiang Province, China
GUO Lan-min
Department of Child Development and Behavior, the Third Affiliated Hospital of Jiamusi University/Children Neurological Rehabilitation Lab, Jiamusi University, Jiamusi 154003, Heilongjiang Province, China
LV Zhi-hai
Department of Child Development and Behavior, the Third Affiliated Hospital of Jiamusi University/Children Neurological Rehabilitation Lab, Jiamusi University, Jiamusi 154003, Heilongjiang Province, China
TIAN Shi-jie
Department of Child Development and Behavior, the Third Affiliated Hospital of Jiamusi University/Children Neurological Rehabilitation Lab, Jiamusi University, Jiamusi 154003, Heilongjiang Province, China
JIANG Zhi-mei
Department of Child Development and Behavior, the Third Affiliated Hospital of Jiamusi University/Children Neurological Rehabilitation Lab, Jiamusi University, Jiamusi 154003, Heilongjiang Province, China
