Laboratory Study
Effects of ubiquitin proteasome inhibitor MG-132 on early embryonic development in mice
Yangyang Lei, Mingrui Li, shuai Zhou, Ran Huo
Published 2018-05-25
Cite as Chin J Reprod Contracep, 2018, 38(5): 386-392. DOI: 10.3760/cma.j.issn.2096-2916.2018.05.008
Abstract
ObjectiveTo investigate the effects of ubiquitin proteasome inhibitor MG-132 on early embryonic development.
MethodsWe collected the embryos of 12 hpi, 24 hpi, 36 hpi, 48 hpi, 60 hpi, 72 hpi, 84 hpi and 96 hpi through in vitro fertilization, the result of Western Blotting showed that polyubiquitinated proteins were mainly accumulated in the embryos of 12 hpi. Based on the above, different concentrations and time gradients were set to determine the optimal concentration and processing time of MG-132 induced cell retardation and delayed in two cell development. Utimately, we found that treatment of embroys with 1 μmol/L MG-132 from14 hpi to18 hpi could lead to retardation and delay in two cell development mostly. Edu assay and immunofluorescence assay were used to observe whether MG-132 could influence the cell cycle of embryos.
ResultsTreatment of embryos with 1 μmol/L MG-132 from 14 hpi to 18 hpi could lead to retardation and delay in two-cell stage development.
ConclusionThe ubiquitin proteasome inhibitor MG-132 could inhibit the degradation of proteins during embryonic development and it could lead to cell cycle of embryonic development arrested in G2/M phase.
Key words:
Ubiquitin proteasome; MG-132; Cell cycle of embryonic development
Contributor Information
Yangyang Lei
Department of Histology and Embryology, Basic Medical College of Nanjing Medical University, Nanjing 211100, China
Mingrui Li
Department of Histology and Embryology, Basic Medical College of Nanjing Medical University, Nanjing 211100, China
shuai Zhou
Department of Histology and Embryology, Basic Medical College of Nanjing Medical University, Nanjing 211100, China
Ran Huo
Department of Histology and Embryology, Basic Medical College of Nanjing Medical University, Nanjing 211100, China
