To investigate the related factors affecting the outcome of testicular microextraction.

The clinical data of 74 patients with non obstructive azoospermia undergoing testicular microsurgery in Family Planning Research Institute of Guangdong Province (Family Planning Hospital of Guangdong Province) from July 2017 to April 2018 were retrospectively analyzed. According to the spermatozoa outcome, they were divided into sperm group (group A) and non-sperm group (group B). The hormone levels, testicular volume, preoperative testicular pathological results, preoperative treatment, plumpness of spermatogenic tubule, blood supply of spermatogenic tubule and interstitial tissue were compared, and the related factors affecting the outcome of testicular microextraction were analyzed.

In 74 cases of non-obstructive azoospermia, 33 cases got sperm, 41 cases did not get sperm, and the sperm acquisition rate was 44.59%. Among them, preoperative treatment, seminiferous tubule plumpness and interstitial tissue hyperplasia in group A were significantly different from those in group B (P<0.05). But there was no difference in hormone levels, testicular volume and blood supply status of seminiferous tubules (P>0.05). Of the 40 patients who had not found sperm before operation, 17 of the patients found sperm, 23 cases did not find sperm, and there was no difference between the results of testicular pathology and the outcome of sperm extraction between those who found the sperm and those who did not find the spermatozoon (P>0.05).

Hormone level, testicular volume, testicular biopsy results, and abundant blood supply of seminiferous tubules do not affect the outcome of testicular microextraction. Spermatozoa are filled with seminiferous tubules and interstitial tissue of middle testis is easy to get spermatozoa.

Microscopic refinement; Influencing factors; Non-obstructive azoospermia