Clinical Report
Effect of the different partial deletions in the AZFc locus of Y-chromosome on the ICSI outcome of severe oligoasthenozoospermia patients
Xia Yanqing, Feng Ke, Qu Xiaowei, Yin Baoli, Xie Juanke, Song Xiaobing, Wan Feng, Zhang Cuilian, Guo Haibin
Published 2022-04-25
Cite as Chin J Reprod Contracep, 2022, 42(4): 388-393. DOI: 10.3760/cma.j.cn101441-20210714-00309
Abstract
ObjectiveTo investigate the effects of different partial deletions in azoospermia factor (AZF) locus of Y-chromosome on the clinical outcome of severe oligoasthenozoospermia patients by intracytoplasmic sperm injection (ICSI).
MethodsA retrospective cohort study was conducted on the patients undergoing high-throughput sequencing for Y chromosome microdeletion screening and ICSI treatment in Reproductive Medicine Center of Henan Provincial People's Hospital from December 2017 to July 2020. According to whether carrying AZFc microdeletions or not, the patients were divided into the AZFc-deletion group and control group. And AZFc-deletion group was divided into 3 subgroups, b2/b3 deletion, b2/b4 deletion and gr/gr deletions subgroup, by the types of partial deletion.
ResultsThe day 3 (D3) available embryo rate, the high-quality embryo rate, and the blastocyst formation rate in patients with AZFc deletion were statistically lower than those in control group [70.4% (556/790) vs. 78.5% (2867/3651), P<0.001; 24.7% (199/807) vs. 34.3% (1284/3747), P<0.001; 51.7% (277/536) vs. 58.0% (1540/2592), P=0.007], and there were no statistical differences in implantation rate, clinical pregnancy rate, live birth rate during transplantation cycle between the two groups (all P>0.05). The AZFc b2/b3 deletion subgroup had no significant differences in D3 available embryo rate, high-quality embryo rate, blastocyst formation rate, implantation rate, clinical pregnancy rate and live birth rate, compared with control group (all P>0.05). The rate of high-quality embryos in patients with the b2/b4 deletion subgroup [23.2% (32/138)] was lower than that of control group (P=0.004), but there were no statistical differences in D3 available embryo rate, blastocyst formation rate, implantation rate, clinical pregnancy rate and live birth rate (all P>0.05). The D3 available embryo rate [71.6% (280/391)], the high-quality embryo rate [20.8% (84/403)] and the blastocyst formation rate [48.7% (133/273)] in patients of gr/gr deletion subgroup were significantly lower than those in control group (P=0.002, P<0.001, P<0.001), but there were no statistical differences in implantation rate, clinical pregnancy rate and live birth rate (all P>0.05).
ConclusionAZFc b2/b3 deletion and b2/b4 deletion in the AZFc locus of Y chromosome have no significant effect on embryonic development and pregnancy outcome in patients with severe oligoasthenozoospermia undergoing ICSI. Gr/gr deletion has most adverse effect on embryonic development but no effect on pregnancy outcome.
Key words:
Y chromosome microdeletion; Azoospermia factor c locus; Severe oligoasthenozoospermia; Sperm microinjection, intracytoplasmic; Clinical outcome
Contributor Information
Xia Yanqing
Reproductive Medicine Center of Henan Provincial People's Hospital, Zhengzhou 450003, China
Feng Ke
Reproductive Medicine Center of Henan Provincial People's Hospital, Zhengzhou 450003, China
Qu Xiaowei
Reproductive Medicine Center of Henan Provincial People's Hospital, Zhengzhou 450003, China
Yin Baoli
Reproductive Medicine Center of Henan Provincial People's Hospital, Zhengzhou 450003, China
Xie Juanke
Reproductive Medicine Center of Henan Provincial People's Hospital, Zhengzhou 450003, China
Song Xiaobing
Reproductive Medicine Center of Henan Provincial People's Hospital, Zhengzhou 450003, China
Wan Feng
Reproductive Medicine Center of Henan Provincial People's Hospital, Zhengzhou 450003, China
Zhang Cuilian
Reproductive Medicine Center of Henan Provincial People's Hospital, Zhengzhou 450003, China
Guo Haibin
Reproductive Medicine Center of Henan Provincial People's Hospital, Zhengzhou 450003, China
