Clinical Study
Analysis of possible factors influencing mosaicism in in vitro fertilization
Liu Jing, Zhou Mengge, Guan Yichun, Li Zhen, Wang Xingling, Zhang Yaxin, Yuan Erfeng, Zhang Linlin, Yang Rujing
Published 2022-09-25
Cite as Chin J Reprod Contracep, 2022, 42(9): 902-908. DOI: 10.3760/cma.j.cn101441-20210519-00222
Abstract
ObjectiveTo preliminarily explore the related factors that affect chimera mosaicism in in vitro fertilization (IVF) treatment.
MethodsA case-control study was conducted to retrospectively analyze the clinical data of 2252 blastocysts in 579 preimplantation genetic testing (PGT) cycles in the Reproductive Medicine Center of the Third Affiliated Hospital of Zhengzhou University from January 2017 to December 2020. Biopsy cells were analyzed by next generation sequencing (NGS). According to the analysis results, all embryos were divided into mosaicism group and non-mosaicism group. Mosaicism types included euploid-aneuploid mosaicism, aneuploid-aneuploid mosaicism and complex mosaicism. The population characteristics and laboratory-related parameters of the two groups of embryos were compared, and single-factor and multi-factor analysis of the incidence of mosaicism were performed to evaluate the related factors that affect the development of mosaic embryos.
ResultsA total of 2252 blastocysts in 579 cycles were included in this study, 905 embryos (40.2%) were euploid, 923 (41.0%) were aneuploid, and 424 (18.8%) were mosaicism. Among them, 228 (10.1%) were euploid-aneuploidy mosaicism, 59 (2.6%) were aneuploidy-aneuploidy mosaicism, and 137 (6.1%) were complex mosaicism. NGS technology was performed in 4 institutions, and the mosaicism rate fluctuated between 7.6% and 26.2%. After adjusting the confounding factors (the age of the male and female partners, the quality of the male partner's sperm, the ovarian stimulation protocols, the type of culture medium, the indications of PGT, the different biopsy operators and the developmental stage of the blastocyst), it was found that the blastocyst trophectoderm cell (TE) score (grade C vs. grade A, P=0.014) and the genetic testing institutions (institution 2 vs. early stage of institution 1, P<0.001; late stage of institution 1 vs. early stage of institution 1, P<0.001) had a significant effect on the occurrence of mosaicism. Compared with the TE score of grade A, the chance of mosaicism in grade C increased by 66% (aOR=1.66, 95% CI=1.11-2.50, P=0.014). Compared with the early stage of institution 1, the incidence of mosaicism in institution 2 and late stage of institution 1 was 2.28 times (aOR=2.28, 95% CI=1.71-3.04, P<0.001), and late stage of institution 1 was 2.17 times that of the early stage (aOR=2.17, 95% CI=1.41-3.34, P<0.001).
ConclusionThe incidence of mosaicism during IVF treatment is related to NGS genetic testing institutions and the quality of trophectoderm cells
Key words:
Mosaicism; Next generation sequencing; Preimplantation genetic testing
Contributor Information
Liu Jing
Reproductive Medicine Center, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Zhou Mengge
Reproductive Medicine Center, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Guan Yichun
Reproductive Medicine Center, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Li Zhen
Reproductive Medicine Center, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Wang Xingling
Reproductive Medicine Center, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Zhang Yaxin
Reproductive Medicine Center, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Yuan Erfeng
Research Center, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Zhang Linlin
Research Center, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
Yang Rujing
Reproductive Medicine Center, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China
