Promoting hospital management is key to high quality development of medical service. In view of the development reality of healthcare, Shenzhen was focused on professionalization and specialization, exploring the implementation path of professional training for hospital administrators. The professional training path consisted of a strict procedure of trainees′ selection, hierarchical and classified curriculum modules setup, as well as program implementation of training and an integral evaluation system. The curriculum modules were designed as a " 5+ 4" system, which referred to five long-term customized courses (i.e., the Party′s construction, government governance, policies and regulations, medical humanities and modern hospital management), and four short-term specialized training courses (i.e., leadership, discipline construction, intelligent hospital and international healthcare). Shenzhen selected 20 trainees from public hospitals and health administrative departments in the city, and held the first professional training course for public hospital presidents in 2020, totalling 336 class hours of systematic training. The 20 trainees scored their general satisfaction over the teaching content and teacher performance of the 5 long-term customized courses as follows. Their scores ranked from high to low were modern hospital management (9.79), policies and regulations (9.75), government governance (9.74), Party′s construction (9.67), and medical humanities (9.64). The score of the trainees′ integral evaluation was (107.2±10.9). This implementation has proved successful, and could provide references for professional training of hospital managers in China.