Clinical Research and Practice
Analysis of newborn screening for galactosemia and genotype-phenotype of confirmed galatosemia cases
Rulai Yang, Fan Tong, Fang Hong, Guling Qian, Dingwen Wu, Zhengyan Zhao
Published 2017-02-02
Cite as Chin J Pediatr, 2017, 55(2): 104-109. DOI: 10.3760/cma.j.issn.0578-1310.2017.02.010
Abstract
ObjectiveTo investigate the prevalence of galactosemia(GAL), and the characteristics of genotype and phenotype of newborns who were confirmed with GAL in newborn screening in Zhejiang province.
MethodThe number of all live births, newborn screened infants and all clinical data of confirmed newborns with GAL from October 2013 to March 2015 were retrospectively analyzed by reviewing the data of Zhejiang Province screening center database. And the characteristics of genes and the clinical data of GAL cases who were confirmed by correlative gene test and enzyme activity measurement were analyzed.
ResultThe prevalence of GAL in Zhejiang province was 1/189 857. Among them, there was 1 case confirmed with GAL typeⅠ (prevalence, 1/759 428), with mutations of c. 904+ 1G>T and c. 687G>A, the enzyme activity of galactose-1-phosphate uridyltransferase (GALT) was 56.4% of controls. And there was 1 case of GAL typeⅡ(prevalence, 1/759 428), with mutations of c. 85G>T and c. 502G>A. There were 2 cases confirmed with GAL type Ⅲ(prevalence, 1/379 714), with mutations of c. 505C>T, c. 452G>A, c. 280G>A and c. 925G>A, the enzyme activity of UDP-galactose-4′-epimerase (GALE) were 42% and 38% of controls, respectively. All cases had different abnormal biochemical marks of liver function, and 1 case had combined hyperlactacidemia or hyperammonemia or increase of multiple kinds of amino acids, respectively. The newborn of GAL type Ⅱ had phacoscotasmus before treatment. All the cases were fed with lactose free milk powder, and all the abnormal parameters were improved during following up.
ConclusionThe disease of GAL is rare in Zhejiang province, and its genotype distribution is scattered with comparatively mind clinical manifestations, and the cases with early treatment with lactose free milk powder have good prognosis. All cases needed to be treated and followed up for a life-long time. It is recommended that the high risk cases with GAL should be screened as soon as possible.
Key words:
Galactosemias; Prevalence; Mutation; Phenotype; Prognosis
Contributor Information
Rulai Yang
Department of Genetic and Metabolic diseases, Children′s Hospital of Zhejiang University School of Medicine, Hangzhou 310052, China
Fan Tong
Fang Hong
Guling Qian
Dingwen Wu
Zhengyan Zhao
