Clinical Research
Expression of nitric oxide in uterine junctional zone of patients with adenomyosis
LIU Jing-jing, DUAN Hua, WANG Sha
Published 2013-07-25
Cite as Chin J Obstet Gynecol, 2013,48(07): 504-507. DOI: 10.3760/cma.j.issn.0529-567x.2013.07.006
Abstract
ObjectiveTo investigate the relationship between nitric oxide and contractile disfunction of junctional zone of smooth muscle by detecting the expression of nitric oxide in uterine junctional zone in patients with adenomyosis.
MethodsFrom February 2012 to July 2012,23 patients with adenomyosis undergoing hysterectomy as adenomyosis group matched as 16 patients with cervical carcinoma or ovarian cancer were enrolled as control group in Center of Minimally Invasive Gynecology,Beijing Obstetrics and Gynecology Hospital.Location of nitric oxide synthase (inducible NOS and endothelial NOS) in uterine junctional zone was analyzed by immunohistochemistry.Smooth muscle cells of junctional zone were cultured and cellular mRNA levels of nitric oxide synthase were detected by real-time polymerase chain reaction.Furthermore,concentration of nitrites in lysates of fresh junctional zone tissues and cultured cells were determined by Griess reaction.
Results (1) The expression of nitric oxide synthase proteins in smooth muscle cells of junctional zone tissues:the staining of iNOS was intensive in smooth muscle cells of junctional zone in patients with adenomyosis,
Key words:
Endometriosis; Myometrium; Nitric Oxide Synthase; Nitric Oxide
Contributor Information
LIU Jing-jing
Center of Minimally Invasive Gynecology,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing 100006,China
DUAN Hua
WANG Sha
